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Nicotinamide clearance by Pnc1 directly regulates Sir2-mediated silencing and longevity

Gallo, C.M.; Smith, D.L.; Smith, J.S.; Mol. Cell. Biol. 24, 1301-1312 (2004)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.19
additional information
stress suppresses the inhibitory effect of nicotinamide through the induction of PNC1 expression
Saccharomyces cerevisiae
Cloned(Commentary)
EC Number
Commentary
Organism
3.5.1.19
gene PNC1, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.19
C167A
site-directed mutagenesis, inactive mutant, no rescue the inhibition of NAD+-dependent histone deacetylase, i.e. HDAC, activity of Sir2 by nicotinamide
Saccharomyces cerevisiae
3.5.1.19
additional information
PNC1 overexpression suppresses the inhibitory effect of exogenously added nicotinamide on silencing, life span, and Hst1-mediated transcriptional repression
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Saccharomyces cerevisiae
nicotinamide is a by-product of the Sir2 deacetylase reaction and is a natural Sir2 inhibitor, Pnc1, therefore, positively regulates Sir2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide during times of stress, PNC1 specifically prevents nicotinamide-induced inhibition of telomeric and rDNA silencing in vivo, overview, the enzyme is involved in the NAD+ biosynthesis and salvage, pathway overview
nicotinate + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.19
Saccharomyces cerevisiae
-
gene PNC1
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.19
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
nicotinamide is a by-product of the Sir2 deacetylase reaction and is a natural Sir2 inhibitor, Pnc1, therefore, positively regulates Sir2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide during times of stress, PNC1 specifically prevents nicotinamide-induced inhibition of telomeric and rDNA silencing in vivo, overview, the enzyme is involved in the NAD+ biosynthesis and salvage, pathway overview
670237
Saccharomyces cerevisiae
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
Pnc1 stimulates Sir2, an NAD+-dependent histone deacetylase, and prevents the accumulation of nicotinamide
670237
Saccharomyces cerevisiae
nicotinate + NH3
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.19
additional information
stress suppresses the inhibitory effect of nicotinamide through the induction of PNC1 expression
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
gene PNC1, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.1.19
C167A
site-directed mutagenesis, inactive mutant, no rescue the inhibition of NAD+-dependent histone deacetylase, i.e. HDAC, activity of Sir2 by nicotinamide
Saccharomyces cerevisiae
3.5.1.19
additional information
PNC1 overexpression suppresses the inhibitory effect of exogenously added nicotinamide on silencing, life span, and Hst1-mediated transcriptional repression
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.1.19
nicotinamide + H2O
Saccharomyces cerevisiae
nicotinamide is a by-product of the Sir2 deacetylase reaction and is a natural Sir2 inhibitor, Pnc1, therefore, positively regulates Sir2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide during times of stress, PNC1 specifically prevents nicotinamide-induced inhibition of telomeric and rDNA silencing in vivo, overview, the enzyme is involved in the NAD+ biosynthesis and salvage, pathway overview
nicotinate + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.19
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.19
nicotinamide + H2O
nicotinamide is a by-product of the Sir2 deacetylase reaction and is a natural Sir2 inhibitor, Pnc1, therefore, positively regulates Sir2-mediated silencing and longevity by preventing the accumulation of intracellular nicotinamide during times of stress, PNC1 specifically prevents nicotinamide-induced inhibition of telomeric and rDNA silencing in vivo, overview, the enzyme is involved in the NAD+ biosynthesis and salvage, pathway overview
670237
Saccharomyces cerevisiae
nicotinate + NH3
-
-
-
?
3.5.1.19
nicotinamide + H2O
Pnc1 stimulates Sir2, an NAD+-dependent histone deacetylase, and prevents the accumulation of nicotinamide
670237
Saccharomyces cerevisiae
nicotinate + NH3
-
-
-
?