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Literature summary extracted from

  • Alonso, G.D.; Schoijet, A.C.; Torres, H.N.; Flawia, M.M.
    TcPDE4, a novel membrane-associated cAMP-specific phosphodiesterase from Trypanosoma cruzi (2006), Mol. Biochem. Parasitol., 145, 40-49.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.53 expression in Escherichia coli Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.53 3-isobutyl-1-methylxanthine
-
 Trypanosoma cruzi
3.1.4.53 dipyridamole
-
 Trypanosoma cruzi
3.1.4.53 EDTA
-
 Trypanosoma cruzi
3.1.4.53 etazolate IC50: 1.3 mM Trypanosoma cruzi
3.1.4.53 papaverine
-
 Trypanosoma cruzi
3.1.4.53 rolipram IC50: 10.46 mM Trypanosoma cruzi
3.1.4.53 trequinsin
-
 Trypanosoma cruzi
3.1.4.53 zaprinast
-
 Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.53 0.02
-
 adenosine 3',5'-cyclic phosphate
-
 Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.53 membrane
-
 Trypanosoma cruzi 16020
-
3.1.4.53 membrane accociated Trypanosoma cruzi 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.53 Mg2+ required Trypanosoma cruzi
3.1.4.53 Mn2+ less efficient than Mg2+ Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.53 Trypanosoma cruzi
-
-
-
3.1.4.53 Trypanosoma cruzi Q4ZHU6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.53 3',5'-cAMP + H2O
-
 Trypanosoma cruzi 5'-AMP
-
 ?
3.1.4.53 adenosine 3',5'-cyclic phosphate + H2O the enzyme is specific fpr cAMP Trypanosoma cruzi adenosine 5'-phosphate
-
 ?
3.1.4.53 additional information no activity with cGMP Trypanosoma cruzi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.53 cAMP-specific phosphodiesterase
-
 Trypanosoma cruzi
3.1.4.53 TcPDE4
-
 Trypanosoma cruzi

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.4.53 0.000146
-
-
 Trypanosoma cruzi dipyridamole
3.1.4.53 0.0013
-
-
 Trypanosoma cruzi etazolate
3.1.4.53 0.01046
-
-
 Trypanosoma cruzi rolipram
3.1.4.53 0.01288
-
-
 Trypanosoma cruzi papaverine
3.1.4.53 0.1262
-
-
 Trypanosoma cruzi 3-isobutyl-1-methylxanthine
3.1.4.53 0.728
-
-
 Trypanosoma cruzi zaprinast
3.1.4.53 1.3
-
 IC50: 1.3 mM Trypanosoma cruzi etazolate
3.1.4.53 1.958
-
-
 Trypanosoma cruzi trequinsin
3.1.4.53 10.46
-
 IC50: 10.46 mM Trypanosoma cruzi rolipram