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Literature summary extracted from
- The Phn system of Mycobacterium smegmatis: a second high-affinity ABC-transporter for phosphate (2006), Microbiology, 152, 3453-3465.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.3.2.1 | additional information | phosphate-limitation induces expression of phnD and pstS | Mycolicibacterium smegmatis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 7.3.2.1 | pst and phn operons, the Phn system is encoded by a three-gene operon, DNA and amino acid sequence determinations and analysis, genomic organization, expression analyis of pstS and phnD, overview | Mycolicibacterium smegmatis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.3.2.1 | additional information | construction of deletion mutants, mutation of either pstS and phnD leads to impaired cell growth in minimal medium, the cells are only viable in high phosphate concentrated medium | Mycolicibacterium smegmatis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.3.2.1 | arsenate | strong inhibition | Mycolicibacterium smegmatis |  |
| 7.3.2.1 | methylphosphonate | competitive, partial inhibition of phosphate transport activity, overview | Mycolicibacterium smegmatis | |
| 7.3.2.1 | phosphite | competitive, partial inhibition of phosphate transport activity, overview | Mycolicibacterium smegmatis | |
| 7.3.2.1 | phosphonate | competitive, partial inhibition of phosphate transport activity, overview | Mycolicibacterium smegmatis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.3.2.1 | additional information | - |
additional information | phosphate transport kinetics in whole wild-type and mutant cells, overview | Mycolicibacterium smegmatis |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.3.2.1 | membrane | - |
Mycolicibacterium smegmatis | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.3.2.1 | Mg2+ | - |
Mycolicibacterium smegmatis | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.3.2.1 | ATP + H2O + phosphate/out | Mycolicibacterium smegmatis | the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview | ADP + phosphate + phosphate/in | - |
? | |
| 7.3.2.1 | ATP + H2O + phosphate/out | Mycolicibacterium smegmatis SG34 | the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview | ADP + phosphate + phosphate/in | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.3.2.1 | Mycolicibacterium smegmatis | - |
genes pstS and phnD | - |
| 7.3.2.1 | Mycolicibacterium smegmatis SG34 | - |
genes pstS and phnD | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.3.2.1 | ATP + H2O + phosphate/out | - |
Mycolicibacterium smegmatis | ADP + phosphate + phosphate/in | - |
? | |
| 7.3.2.1 | ATP + H2O + phosphate/out | the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview | Mycolicibacterium smegmatis | ADP + phosphate + phosphate/in | - |
? | |
| 7.3.2.1 | ATP + H2O + phosphate/out | - |
Mycolicibacterium smegmatis SG34 | ADP + phosphate + phosphate/in | - |
? | |
| 7.3.2.1 | ATP + H2O + phosphate/out | the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview | Mycolicibacterium smegmatis SG34 | ADP + phosphate + phosphate/in | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.3.2.1 | high-affinity phosphate ABC-transporter | - |
Mycolicibacterium smegmatis |
| 7.3.2.1 | Phn | - |
Mycolicibacterium smegmatis |
| 7.3.2.1 | Pst | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 7.3.2.1 | 37 | - |
assay at | Mycolicibacterium smegmatis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.3.2.1 | 7.5 | - |
assay at | Mycolicibacterium smegmatis |
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