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Literature summary extracted from

  • Cahyanto, M.N.; Kawasaki, H.; Nagashio, M.; Fujiyama, K.; Seki, T.
    Regulation of aspartokinase, aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase and dihydrodipicolinate reductase in Lactobacillus plantarum (2006), Microbiology, 152, 105-112.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.1.11 biotechnology development of lysine-overproducing strains Lactiplantibacillus plantarum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.3 expression in Escherichia coli Lactiplantibacillus plantarum
1.2.1.11 expression in Escherichia coli Hfr3000 Lactiplantibacillus plantarum
1.2.1.11 genes are amplified from chromosomal DNA of Lactobacillus plantarum and cloned into the pCR2.1-TOPO vector for sequencing, and subcloned into the pUC18 vector for heterologous expression in Escherichia coli Lactiplantibacillus plantarum
2.7.2.4 into vector pCR2.1-TOPO, subcloned to pUC18 for heterologous expression of thrA1 or thrA2 in Escherichia coli Gif 102 Lactiplantibacillus plantarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.3 additional information Thr does not inhibit homoserine dehydrogenase 1 Lactiplantibacillus plantarum
1.1.1.3 Thr 90% inhibition of homoserine dehydrogenase 2 at 10 mM Lactiplantibacillus plantarum
1.2.1.11 petrosamine B 50% inhibition at 0.306 mM. Petrosamine B is a pyridoacridine alkaloid isolated from the sponge Oceanapia sp. Lactiplantibacillus plantarum
1.17.1.8 additional information no regulation of enzyme by amino acids from the aspartate family Lactiplantibacillus plantarum
2.7.2.4 L-isoleucine
-
 Lactiplantibacillus plantarum
2.7.2.4 L-lysine inhibits the activity of ThrA2 by 87% at 1 mM and by 96% at 10 mM, no concerted inhibition by L-lysine plus L-threonine Lactiplantibacillus plantarum
2.7.2.4 L-methionine
-
 Lactiplantibacillus plantarum
2.7.2.4 L-threonine represses, reduces growth rate, no concerted inhibition by L-lysine plus L-threonine Lactiplantibacillus plantarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.4 Mg2+
-
 Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+ Lactiplantibacillus plantarum
-
 L-4-aspartyl phosphate + NADPH + H+
-
 ?
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+ Lactiplantibacillus plantarum NCIMB 8826
-
 L-4-aspartyl phosphate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.3 Lactiplantibacillus plantarum
-
-
-
1.2.1.11 Lactiplantibacillus plantarum
-
-
-
1.2.1.11 Lactiplantibacillus plantarum NCIMB 8826
-
-
-
1.17.1.8 Lactiplantibacillus plantarum
-
-
-
2.7.2.4 Lactiplantibacillus plantarum
-
-
-
2.7.2.4 Lactiplantibacillus plantarum NCIMB 8826
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.11 cell culture weakly expressed Lactiplantibacillus plantarum
-
1.2.1.11 cell culture highly expressed Lactiplantibacillus plantarum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.11 0.215
-
 gene asd1 Lactiplantibacillus plantarum
1.2.1.11 2.149
-
 gene asd2 Lactiplantibacillus plantarum
1.2.1.11 2.149
-
 when expressed in Escherichia coli Lactiplantibacillus plantarum
1.17.1.8 1.153
-
 pH 7.5, 30°C, recombinant enzyme Lactiplantibacillus plantarum
2.7.2.4 0.00036
-
 thrA1 Lactiplantibacillus plantarum
2.7.2.4 0.00096
-
 thrA2, in the presence of L-lysine Lactiplantibacillus plantarum
2.7.2.4 0.162
-
 thrA2 Lactiplantibacillus plantarum
2.7.2.4 0.196
-
 thrA2, in the presence of L-threonine Lactiplantibacillus plantarum
2.7.2.4 0.289
-
 thrA2, in the presence of L-isoleucine Lactiplantibacillus plantarum
2.7.2.4 0.29
-
 thrA2, in the presence of L-methionine Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.3 L-aspartate 4-semialdehyde + NADPH + H+
-
 Lactiplantibacillus plantarum L-homoserine + NADP+
-
 ?
1.1.1.3 L-aspartate 4-semialdehyde + NADPH + H+
-
 Lactiplantibacillus plantarum NCIMB 8826 L-homoserine + NADP+
-
 ?
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Lactiplantibacillus plantarum L-4-aspartyl phosphate + NADPH + H+
-
 ?
1.2.1.11 L-aspartate 4-semialdehyde + phosphate + NADP+
-
 Lactiplantibacillus plantarum NCIMB 8826 L-4-aspartyl phosphate + NADPH + H+
-
 ?
1.17.1.8 2,3-dihydrodipicolinate + NAD(P)H
-
 Lactiplantibacillus plantarum 2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
 ?
1.17.1.8 2,3-dihydrodipicolinate + NAD(P)H
-
 Lactiplantibacillus plantarum NCIMB 8826 2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
 ?
2.7.2.4 ATP + L-aspartate
-
 Lactiplantibacillus plantarum ADP + 4-phospho-L-aspartate
-
 ?
2.7.2.4 ATP + L-aspartate
-
 Lactiplantibacillus plantarum NCIMB 8826 ADP + 4-phospho-L-aspartate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.3 homoserine dehydrogenase 1
-
 Lactiplantibacillus plantarum
1.1.1.3 homoserine dehydrogenase 2
-
 Lactiplantibacillus plantarum
1.2.1.11 ASADHD
-
 Lactiplantibacillus plantarum
1.2.1.11 Asd1
-
 Lactiplantibacillus plantarum
1.2.1.11 Asd2
-
 Lactiplantibacillus plantarum
1.2.1.11 aspartate semialdehyde dehydrogenase
-
 Lactiplantibacillus plantarum
1.2.1.11 aspartate-semialdehyde dehydrogenase
-
 Lactiplantibacillus plantarum
2.7.2.4 AK
-
 Lactiplantibacillus plantarum
2.7.2.4 aspartokinase
-
 Lactiplantibacillus plantarum
2.7.2.4 thrA1
-
 Lactiplantibacillus plantarum
2.7.2.4 thrA2
-
 Lactiplantibacillus plantarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.11 30
-
 activity assay Lactiplantibacillus plantarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.11 9
-
 activity assay Lactiplantibacillus plantarum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.3 NADPH
-
 Lactiplantibacillus plantarum
1.2.1.11 NADPH
-
 Lactiplantibacillus plantarum
1.17.1.8 NAD(P)H
-
 Lactiplantibacillus plantarum