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Literature summary extracted from
- Mutational effect for stability in a conserved region of thermolysin (2005), Lett. Appl. Microbiol., 40, 329-334.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.24.27 | DELTA127 | absence of CaCl2, 18% of wild-type activity, presence of 5 mM CaCl2, 71% of wild-type activity. Decrease in amount of enzyme secreted compared to wild-type | Bacillus thermoproteolyticus |
| 3.4.24.27 | L155A | absence of CaCl2, 87% of wild-type activity, presence of 5 mM CaCl2, 83% of wild-type activity. Amount of enzyme secreted is about the same level as wild-type | Bacillus thermoproteolyticus |
| 3.4.24.27 | M205P | absence of CaCl2, 0.52% of wild-type activity, presence of 5 mM CaCl2, 48% of wild-type activity. Decrease in amount of enzyme secreted compared to wild-type | Bacillus thermoproteolyticus |
| 3.4.24.27 | P208H | absence of CaCl2, 0.61% of wild-type activity, presence of 5 mM CaCl2, 61% of wild-type activity. Decrease in amount of enzyme secreted compared to wild-type | Bacillus thermoproteolyticus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.27 | Bacillus thermoproteolyticus | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.27 | 281 | - |
pH 7.0 | Bacillus thermoproteolyticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.27 | azocasein + H2O | - |
Bacillus thermoproteolyticus | ? | - |
? |  |
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Release 2023.1 (January 2023)
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