Any feedback?
Literature summary extracted from
- Chlorella virus-encoded deoxyuridine triphosphatases exhibit different temperature optima (2005), J. Virol., 79, 9945-9953.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.6.1.23 | expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Paramecium bursaria Chlorella virus 1 |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.23 | dUTP | substrate inhibition at higher concentrations | Paramecium bursaria Chlorella virus 1 |  |
| 3.6.1.23 | additional information | no inhibition by dTTp, dGTP, dATP, and dCTP | Paramecium bursaria Chlorella virus 1 |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.23 | 0.0117 | - |
dUTP | pH 8.5, 50°C, recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.23 | Mg2+ | best divalent cation, bound at the center of the trimeric enzyme structure | Paramecium bursaria Chlorella virus 1 | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.23 | 54000 | - |
recombinant His-tagged enzyme, native PAGE | Paramecium bursaria Chlorella virus 1 |
| 3.6.1.23 | 176000 | - |
3 * 176000, recombinant His-tagged enzyme, SDS-PAGE | Paramecium bursaria Chlorella virus 1 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.23 | dUTP + H2O | Paramecium bursaria Chlorella virus 1 | dUMP is required for biosynthesis of dTTP, overview | dUMP + diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.23 | Paramecium bursaria Chlorella virus 1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.23 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Paramecium bursaria Chlorella virus 1 |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.6.1.23 | dUTP + H2O = dUMP + diphosphate | active site structure, R84 and S81 are involved in substrate binding, molecular modeling | Paramecium bursaria Chlorella virus 1 |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.23 | additional information | - |
- |
Paramecium bursaria Chlorella virus 1 |
| 3.6.1.23 | 23.9 | - |
purified recombinant enzyme | Paramecium bursaria Chlorella virus 1 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.23 | dUTP + H2O | dUMP is required for biosynthesis of dTTP, overview | Paramecium bursaria Chlorella virus 1 | dUMP + diphosphate | - |
? | |
| 3.6.1.23 | dUTP + H2O | the enzyme is highly specific for dUTP | Paramecium bursaria Chlorella virus 1 | dUMP + diphosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.6.1.23 | More | molecular structure modeling | Paramecium bursaria Chlorella virus 1 |
| 3.6.1.23 | trimer | 3 * 176000, recombinant His-tagged enzyme, SDS-PAGE | Paramecium bursaria Chlorella virus 1 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.6.1.23 | deoxyuridine triphosphatase | - |
Paramecium bursaria Chlorella virus 1 |
| 3.6.1.23 | dUTPase | - |
Paramecium bursaria Chlorella virus 1 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.23 | 50 | - |
- |
Paramecium bursaria Chlorella virus 1 |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.23 | 25 | 65 | active range | Paramecium bursaria Chlorella virus 1 |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.23 | 6.8 | - |
dUTP | pH 8.5, 50°C, recombinant enzyme | Paramecium bursaria Chlorella virus 1 | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.23 | 8.5 | - |
- |
Paramecium bursaria Chlorella virus 1 |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.23 | 2 | 11 | active range | Paramecium bursaria Chlorella virus 1 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
