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Literature summary extracted from

  • Pavlova, M.; Klva?a, M.; Jesenska, A.; Prokop, Z.; Kone?na, H.; Sato, T.; Tsuda, M.; Nagata, Y.; Damborsky, J.
    The identification of catalytic pentad in the haloalkane dehalogenase DhmA from Mycobacterium avium N85: Reaction mechanism and molecular evolution (2006), J. Struct. Biol., 157, 384-392.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.5 the coding sequence is amplified in the pAL-781-dhmA vector, the vector pUC18-dhmAHis is propagated in Escherichia coli JM110, the dhmAHis gene is ligated into the vector pK4RP for expression in Rhodococcus erythropolis Mycobacterium avium

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.5 D123A no enzymic activity Mycobacterium avium
3.8.1.5 D123A mutant to verify catalytic relevant residues, no statistically significant activity is detected Mycobacterium avium
3.8.1.5 D250A no enzymic activity Mycobacterium avium
3.8.1.5 D250A mutant to verify catalytic relevant residues, no statistically significant activity is detected Mycobacterium avium
3.8.1.5 H279A no enzymic activity Mycobacterium avium
3.8.1.5 H279A mutant to verify catalytic relevant residues, no statistically significant activity is detected Mycobacterium avium
3.8.1.5 W124L no enzymic activity Mycobacterium avium
3.8.1.5 W124L mutant to verify catalytic relevant residues, no statistically significant activity is detected Mycobacterium avium
3.8.1.5 W164L no enzymic activity Mycobacterium avium
3.8.1.5 W164L mutant to verify catalytic relevant residues, no statistically significant activity is detected Mycobacterium avium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.5 36000
-
 determined by SDS-PAGE Mycobacterium avium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.5 1-haloalkane + H2O Mycobacterium avium
-
 a primary alcohol + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Mycobacterium avium
-
 expression in Rhodococcus erythropolis IAM 1399
-
3.8.1.5 Mycobacterium avium Q73Y99
-
-
3.8.1.5 Mycobacterium avium N85
-
 expression in Rhodococcus erythropolis IAM 1399
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.5 the cell free extract of Rhodococcus erythropolis IAM1399 cells is purified on a BD TALON metal affinity resin Mycobacterium avium

Reaction

EC Number Reaction Comment Organism Reaction ID
3.8.1.5 1-haloalkane + H2O = a primary alcohol + halide catalytic triad consists of D123, H279, D250, with a first halide-stabilizing W124 and a secong stabilizing residue W164. Two-step reaction mechanism Mycobacterium avium
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.5 0.00066
-
 1,2-dichloroethane Mycobacterium avium
3.8.1.5 0.6
-
 1-bromobutane Mycobacterium avium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 1,2-dichloroethane + H2O
-
 Mycobacterium avium ?
-
 ?
3.8.1.5 1-bromobutane + H2O
-
 Mycobacterium avium n-butanol + bromide
-
 ?
3.8.1.5 1-bromobutane + H2O
-
 Mycobacterium avium 1-butanol + bromide
-
 ?
3.8.1.5 1-bromobutane + H2O
-
 Mycobacterium avium N85 1-butanol + bromide
-
 ?
3.8.1.5 1-haloalkane + H2O
-
 Mycobacterium avium a primary alcohol + halide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.8.1.5 DhmA
-
 Mycobacterium avium
3.8.1.5 haloalkane dehalogenase
-
 Mycobacterium avium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.5 20
-
 activity assay with 1,2-dichloroethane Mycobacterium avium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.5 8.6
-
 activity assay with 1,2-dichloroethane Mycobacterium avium
3.8.1.5 8.6
-
 activity assay with 1-bromobutane Mycobacterium avium