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Literature summary extracted from

  • Yildirim, S.; Ruinatscha, R.; Gross, R.; Wohlgemuth, R.; Kohler, H.E.; Witholt, B.; Schmid, A.
    Selective hydrolysis of the nitrile group of cis-dihydrodiols from aromatic nitriles (2006), J. Mol. Catal. B, 38, 76-83.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.5.1 synthesis biotransformation of trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile to trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylic acid with isolated enzyme, immobilized enzyme and with recombinant cells containing AtNIT1 Arabidopsis thaliana
3.5.5.1 synthesis biotransformation of trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile to trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylic acid with isolated enzyme, immobilized enzyme and with recombinant cells containing AtNIT1 Rhodococcus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expression in Escherichia coli Arabidopsis thaliana
3.5.5.1 expression in Escherichia coli Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Arabidopsis thaliana
-
-
-
3.5.5.1 Rhodococcus sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 cis-1,2-dihydroxy-3-cyanocyclohexa-3,5-diene + H2O
-
 Rhodococcus sp. 5,6-dihydroxycyclohexa-1,3-diene-1-carboxylic acid + NH3
-
 ?
3.5.5.1 trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile + H2O
-
 Arabidopsis thaliana trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylic acid + NH3
-
 ?
3.5.5.1 trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylonitrile + H2O
-
 Rhodococcus sp. trans-3-[(5S,6R)-5,6-dihydroxycyclohexa-1,3-dienyl]-acrylic acid + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 nitrilase AtNIT1
-
 Arabidopsis thaliana
3.5.5.1 nitrilase AtNIT1
-
 Rhodococcus sp.