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Literature summary extracted from

  • Kamitori, S.; Iguchi, A.; Ohtaki, A.; Yamada, M.; Kita, K.
    X-ray structures of NADPH-dependent carbonyl reductase from Sporobolomyces salmonicolor provide insights into stereoselective reductions of carbonyl compounds (2005), J. Mol. Biol., 352, 551-558.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 expression of wild-type enzyme in Escherichia coli strain JM109, and of selenomethionine-CR in Escherichia coli strain B834 Sporidiobolus salmonicolor

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.184 purified recombinant free wild-type or selenomethionine-labeled enzyme and in complex with NADPH, vapour disffusion method, 30 mg/ml protein in 20 mM Tris-HCl, pH 8.0, against a reservoir solution containing 32% w/v PEG 2000 monomethyl ether, 100 mM ammonium sulfate, and 0.2 M sodium acetate, pH 5.0, with or without 4 mM NADPH, X-ray diffraction structure determination and analysis at 1.8 A and 1.6 A resolution, respectively, structure modeling Sporidiobolus salmonicolor

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Sporidiobolus salmonicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 recombinant wild-type enzyme from Escherichia coli strain JM109 and recombinant selenomethionine-CR from Escherichia coli strain B834, by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography, and gel filtration Sporidiobolus salmonicolor

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.184 R-CHOH-R' + NADP+ = R-CO-R' + NADPH + H+ stereoselective enzyme, reaction mechanism and substrate binding model, formation of a hydrophobic channel induced by NADPH binding Sporidiobolus salmonicolor
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 benzaldehyde + NADPH + H+
-
 Sporidiobolus salmonicolor benzylalcohol + NADP+
-
 ?
1.1.1.184 camphorquinone + NADPH + H+
-
 Sporidiobolus salmonicolor ?
-
 ?
1.1.1.184 ethyl 4-chloro-3-oxobutanoate + NAD(P)H + H+ stereospecific reaction, formation of a hydrophobic channel induced by NADPH binding, structure overview Sporidiobolus salmonicolor ethyl (S)-4-chloro-3-hydroxybutanoate + NAD(P)+ optically pure (S)-enantiomer ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 More SSCR has two domains, an NADPH-binding domain and a substrate-binding domain, structure overview Sporidiobolus salmonicolor

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 More the enzyme belongs to the short-chain dehydrogenases/reductases family Sporidiobolus salmonicolor
1.1.1.184 SSCR
-
 Sporidiobolus salmonicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADPH dependent on, structure of the NADPH-binding domain and interaction between the enzyme and NADPH, overview Sporidiobolus salmonicolor