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Literature summary extracted from

  • Schmidt, A.E.; Stewart, J.E.; Mathur, A.; Krishnaswamy, S.; Bajaj, S.P.
    Na+ site in blood coagulation factor IXa: effect on catalysis and factor VIIIa binding (2005), J. Mol. Biol., 350, 78-91.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.22 factor FVIIa in complex with tissue factor and Ca2+ Homo sapiens
3.4.21.22 factor FVIIIa
-
 Homo sapiens
3.4.21.22 factor FXIa in presence of 5 mM CaCl2 Homo sapiens
3.4.21.22 Phospholipid provided by platelet surface Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.22 expressed in HEK293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.22 FIXagamma225P mutant enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.22 Ca2+ 5 mM Homo sapiens
3.4.21.22 Na+ 200 mM in the absence of Ca2+, 185 mM in the presence of 5 mM Ca2+ Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.22 0.5
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa, in presence of 200 mM Na+ Homo sapiens
3.4.21.22 1.9
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa Homo sapiens
3.4.21.22 2.1
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P Homo sapiens
3.4.21.22 3.1
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P, in presence of 200 mM Na+ Homo sapiens
3.4.21.22 4.5
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa, in presence of 5 mM Ca2+ and 185 mM Na+ Homo sapiens
3.4.21.22 7.3
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P, in presence of 5 mM Ca2+ and 185 mM Na+ Homo sapiens
3.4.21.22 7.9
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P, in presence of 200 mM Ca2+ Homo sapiens
3.4.21.22 8.7
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa, in presence of 200 mM Ca2+ Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.22 Ca2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.22 57000
-
 SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.22 Homo sapiens P00740
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.22 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.22 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens 4-methylsulfonyl-D-Leu-Gly-Arg + p-nitroaniline
-
 ?
3.4.21.22 factor X + H2O
-
 Homo sapiens factor Xa
-
 ?
3.4.21.22 p-aminobenzamidine + H2O
-
 Homo sapiens p-aminobenzoic acid + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.22 factor IXa
-
 Homo sapiens
3.4.21.22 FIXa
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.22 0.005
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P Homo sapiens
3.4.21.22 0.058
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P, in presence of 200 mM Na+ Homo sapiens
3.4.21.22 0.42
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa Homo sapiens
3.4.21.22 0.63
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa, in presence of 200 mM Na+ Homo sapiens
3.4.21.22 0.78
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P, in presence of Ca2+ and 185 mM Na+ Homo sapiens
3.4.21.22 0.89
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P, in presence of 200 mM Ca2+ Homo sapiens
3.4.21.22 3.15
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa, in presence of 200 mM Ca2+ Homo sapiens
3.4.21.22 3.38
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa, in presence of Ca2+ and 185 mM Na+ Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.22 0.025
-
 Na+ wild type enzyme FIXa, in the presence of 200 mM Na+ Homo sapiens
3.4.21.22 0.099
-
 Na+ wild type enzyme FIXa Homo sapiens
3.4.21.22 0.119
-
 Na+ wild type enzyme FIXa, in the presence of 185 mM Na+ and 5 mM Ca2+ Homo sapiens
3.4.21.22 0.166
-
 Na+ wild type enzyme FIXa, in the presence of 5 mM Ca2+ Homo sapiens
3.4.21.22 0.179
-
 Na+ mutant enzyme FIXagamma225P, in the presence of 185 mM Na+ and 5 mM Ca2+ Homo sapiens
3.4.21.22 0.184
-
 Na+ mutant enzyme FIXagamma225P, in the presence of 5 mM Ca2+ Homo sapiens
3.4.21.22 0.813
-
 Na+ mutant enzyme FIXagamma225P, in the presence of 200 mM Na+ Homo sapiens