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Literature summary extracted from

  • Radha Kishan, K.V.; Vohra, R.M.; Ganesan, K.; Agrawal, V.; Sharma, V.M.; Sharma, R.
    Molecular structure of D-hydantoinase from Bacillus sp. AR9: evidence for mercury inhibition (2005), J. Mol. Biol., 347, 95-105.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.2 expression in Escherichia coli JM109 Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.2
-
 Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.2 1,10-phenanthroline at 1 mM 47% and at 2 mM 46% residual enzyme acitvity Bacillus sp. (in: Bacteria)
3.5.2.2 diethylpyrocarbonate at 2 mM 95% and at 5 mM 80% residual enzyme acitvity Bacillus sp. (in: Bacteria)
3.5.2.2 EDTA at 5 mM 81% and at 10 mM 71% residual enzyme acitvity Bacillus sp. (in: Bacteria)
3.5.2.2 Mercuric chloride 1 mM completely abrogates activity Bacillus sp. (in: Bacteria)
3.5.2.2 additional information not inhibited by N-ethylmaleimide, iodoacetamide, iodoacetic acid, PMSF, phenylglyoxal Bacillus sp. (in: Bacteria)
3.5.2.2 N-bromosuccinimide 1 mM completely abrogates activity Bacillus sp. (in: Bacteria)
3.5.2.2 p-hydroxymercuric benzoate 1 mM completely abrogates activity Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.2 Al3+ at 2 mM 112% relative enzyme activity Bacillus sp. (in: Bacteria)
3.5.2.2 Ca2+ at 2 mM 123% relative enzyme activity Bacillus sp. (in: Bacteria)
3.5.2.2 Co2+ at 2 mM 162% relative enzyme activity Bacillus sp. (in: Bacteria)
3.5.2.2 Cu2+ at 2 mM 103% relative enzyme activity Bacillus sp. (in: Bacteria)
3.5.2.2 Hg2+ at 2 mM 0% relative enzyme activity Bacillus sp. (in: Bacteria)
3.5.2.2 Mg2+ at 2 mM 116% relative enzyme activity Bacillus sp. (in: Bacteria)
3.5.2.2 Mn2+ at 2 mM 116% relative enzyme activity, two manganese ions modelled in the active site Bacillus sp. (in: Bacteria)
3.5.2.2 additional information non metal ions, 100% relative enzyme activity Bacillus sp. (in: Bacteria)
3.5.2.2 Ni2+ at 2 mM 124% relative enzyme activity Bacillus sp. (in: Bacteria)
3.5.2.2 Zn2+ at 2 mM 118% relative enzyme activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.2 210000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Bacillus sp. (in: Bacteria)
-
 AR9
-
3.5.2.2 Bacillus sp. (in: Bacteria) AR9
-
 AR9
-
3.5.2.2 Geobacillus stearothermophilus
-
-
-
3.5.2.2 Paenarthrobacter aurescens
-
-
-
3.5.2.2 Ralstonia pickettii
-
-
-
3.5.2.2 Thermus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrouracil + H2O 26% relative enzyme activity Bacillus sp. (in: Bacteria) 3-ureidopropanoate
-
 ?
3.5.2.2 5,6-dihydrouracil + H2O 26% relative enzyme activity Bacillus sp. (in: Bacteria) AR9 3-ureidopropanoate
-
 ?
3.5.2.2 DL-p-hydroxyphenylhydantoin + H2O 19% relative enzyme activity Bacillus sp. (in: Bacteria) N-carbamoyl-D-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-p-hydroxyphenylhydantoin + H2O 19% relative enzyme activity Bacillus sp. (in: Bacteria) AR9 N-carbamoyl-D-hydroxyphenylglycine
-
 ?
3.5.2.2 DL-phenylhydantoin + H2O 39.9% relative enzyme activity Bacillus sp. (in: Bacteria) N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 DL-phenylhydantoin + H2O 39.9% relative enzyme activity Bacillus sp. (in: Bacteria) AR9 N-carbamoyl-D-phenylglycine
-
 ?
3.5.2.2 hydantoin + H2O preferred substrate, 100% relative enzyme activity Bacillus sp. (in: Bacteria) N-carbamoyl glycine
-
 ?
3.5.2.2 hydantoin + H2O preferred substrate, 100% relative enzyme activity Bacillus sp. (in: Bacteria) AR9 N-carbamoyl glycine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.2 monomer recombinant enzyme expressed in Escherichia coli Bacillus sp. (in: Bacteria)
3.5.2.2 tetramer purified enzyme from the native Bacillus sp. AR9 by gel filtration Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
3.5.2.2 aaHYD
-
 Paenarthrobacter aurescens
3.5.2.2 bar9HYD
-
 Bacillus sp. (in: Bacteria)
3.5.2.2 bpHYD
-
 Ralstonia pickettii
3.5.2.2 bsHYD
-
 Geobacillus stearothermophilus
3.5.2.2 D-hydantoinase
-
 Bacillus sp. (in: Bacteria)
3.5.2.2 hydantoinase
-
 Geobacillus stearothermophilus
3.5.2.2 hydantoinase
-
 Thermus sp.
3.5.2.2 hydantoinase
-
 Ralstonia pickettii
3.5.2.2 hydantoinase
-
 Paenarthrobacter aurescens
3.5.2.2 thHYD
-
 Thermus sp.