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Literature summary extracted from

  • Kim, J.; Yu, T.S.
    Purification and properties of intracellular cytosine deaminase from Chromobacterium violaceum YK 391 (2004), J. Microbiol. Biotechnol., 14, 1182-1189.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.1 2,2'-dipyridyl
-
 Chromobacterium violaceum
3.5.4.1 2-thiocytosine weak Chromobacterium violaceum
3.5.4.1 2-Thiouracil strong Chromobacterium violaceum
3.5.4.1 5-Azacytosine weak Chromobacterium violaceum
3.5.4.1 chloramine-T
-
 Chromobacterium violaceum
3.5.4.1 Cu2+ strong Chromobacterium violaceum
3.5.4.1 Fe3+ strong Chromobacterium violaceum
3.5.4.1 Hg2+ strong Chromobacterium violaceum
3.5.4.1 N-bromosuccinimide
-
 Chromobacterium violaceum
3.5.4.1 o-phenanthroline
-
 Chromobacterium violaceum
3.5.4.1 p-chloromercuribenzoate
-
 Chromobacterium violaceum
3.5.4.1 Pb2+ strong Chromobacterium violaceum
3.5.4.1 Zn2+ strong Chromobacterium violaceum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.1 0.38
-
 cytosine pH 7.5, 30°C Chromobacterium violaceum
3.5.4.1 0.87
-
 5-fluorocytosine pH 7.5, 30°C Chromobacterium violaceum
3.5.4.1 2.32
-
 5-Methylcytosine pH 7.5, 30°C Chromobacterium violaceum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.1 63000
-
 2 * 63000, SDS-PAGE Chromobacterium violaceum
3.5.4.1 126000
-
-
 Chromobacterium violaceum

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.1 Chromobacterium violaceum
-
 intracellular isoform
-
3.5.4.1 Chromobacterium violaceum YK 391
-
 intracellular isoform
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.1
-
 Chromobacterium violaceum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.1 5-fluorocytosine + H2O
-
 Chromobacterium violaceum 5-fluorouracil + NH3
-
 ?
3.5.4.1 5-fluorocytosine + H2O
-
 Chromobacterium violaceum YK 391 5-fluorouracil + NH3
-
 ?
3.5.4.1 5-methylcytosine + H2O
-
 Chromobacterium violaceum thymine + NH3
-
 ?
3.5.4.1 5-methylcytosine + H2O
-
 Chromobacterium violaceum YK 391 thymine + NH3
-
 ?
3.5.4.1 6-azacytosine + H2O
-
 Chromobacterium violaceum 6-azauracil + NH3
-
 ?
3.5.4.1 6-azacytosine + H2O
-
 Chromobacterium violaceum YK 391 6-azauracil + NH3
-
 ?
3.5.4.1 cytidine + H2O
-
 Chromobacterium violaceum 1-beta-D-ribofuranosylpyrimidin-2(1H)-one + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Chromobacterium violaceum uracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Chromobacterium violaceum YK 391 uracil + NH3
-
 ?
3.5.4.1 additional information no substrate: 5-azacytosine Chromobacterium violaceum ?
-
 ?
3.5.4.1 additional information no substrate: 5-azacytosine Chromobacterium violaceum YK 391 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.1 dimer 2 * 63000, SDS-PAGE Chromobacterium violaceum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.1 30
-
-
 Chromobacterium violaceum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.1 30
-
 stable for a week Chromobacterium violaceum
3.5.4.1 60
-
 5 min, 70% residual activity Chromobacterium violaceum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.1 7.5
-
-
 Chromobacterium violaceum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.4.1 6 8
-
 Chromobacterium violaceum