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Literature summary extracted from

  • Kim, J.I.; Lee, S.M.; Jung, H.J.
    Characterization of calcium-activated bifunctional peptidase of the psychrotrophic Bacillus cereus (2005), J. Microbiol., 43, 237-243.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.57 1,10-phenanthroline 20% inhibition at 0.01 mM, 55% inhibition at 0.1 mM, 64% inhibition at 0.5 mM Bacillus cereus
3.4.21.57 bestatin 6% inhibition at 0.001 mM, 39% inhibition at 0.005 mM, 54% inhibition at 0.01 mM, 57% inhibition at 0.1 mM Bacillus cereus
3.4.21.57 Cu2+ 87% inhibition at 1 mM Bacillus cereus
3.4.21.57 EDTA 45% inhibition at 0.01 mM, 64% inhibition at 0.1 mM, 84% inhibition at 0.5 mM Bacillus cereus
3.4.21.57 EGTA 17% inhibition at 0.01 mM, 44% inhibition at 0.1 mM, 44% inhibition at 0.5 mM Bacillus cereus
3.4.21.57 Fe2+ 100% inhibition at 0.1 mM Bacillus cereus
3.4.21.57 phenylmethylsulfonyl fluoride 67% inhibition at 0.01 mM, 100% inhibition above 0.1 mM Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.57 0.315
-
 N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide 50 mM Tris-HCl pH 8.0, 37°C, 10 min Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.57 Ca2+ 86% activation at 1 mM Bacillus cereus
3.4.21.57 Mg2+ 56% activation at 1 mM Bacillus cereus
3.4.21.57 Mn2+ 39% activation at 1 mM Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.57 Bacillus cereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.57 to homogeneity Bacillus cereus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.57 cell culture
-
 Bacillus cereus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.57 N-succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O
-
 Bacillus cereus N-succinyl-Ala-Ala-Pro-Leu + p-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.57 Leucine endopeptidase
-
 Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.57 60
-
-
 Bacillus cereus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.57 10 70 30% of activity at 10°C and 70°C Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.57 70
-
 complete loss of acivity after 30 min at 70°C Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.57 9
-
-
 Bacillus cereus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.57 6 11
-
 Bacillus cereus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.57 0.0067
-
 bestatin 50 mM Tris-HCl pH 8.0, 37°C, 10 min Bacillus cereus