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Literature summary extracted from

  • Wakayama, M.; Yamagata, T.; Kamemura, A.; Bootim, N.; Yano, S.; Tachiki, T.; Yoshimune, K.; Moriguchi, M.
    Characterization of salt-tolerant glutaminase from Stenotrophomonas maltophilia NYW-81 and its application in Japanese soy sauce fermentation (2005), J. Ind. Microbiol. Biotechnol., 32, 383-390.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.2 nutrition the enzyme is applicated in Japanese soy sauce fermentation Stenotrophomonas maltophilia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 CoCl2 14% inhibition at 1 mM Stenotrophomonas maltophilia
3.5.1.2 CuCl2 20% inhibition at 1 mM, 17% at 0.1 mM Stenotrophomonas maltophilia
3.5.1.2 EDTA 10% inhibition at 1 mM Stenotrophomonas maltophilia
3.5.1.2 MgCl2 20% inhibition at 1 mM, 12% at 0.1 mM Stenotrophomonas maltophilia
3.5.1.2 MnCl2 22% inhibition at 1 mM, 13% at 0.1 mM Stenotrophomonas maltophilia
3.5.1.2 ZnCl2 7% inhibition at 1 mM Stenotrophomonas maltophilia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.2 KCl 25% activation at 1 mM Stenotrophomonas maltophilia
3.5.1.2 additional information the enzyme is not affected by NiCl2 at 1 mM Stenotrophomonas maltophilia
3.5.1.2 NaCl the enzyme is salt-tolerant, over 86% of maximal activity at 16% NaCl, no effect at 1 mM Stenotrophomonas maltophilia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.2 36000
-
 1 * 36000, SDS-PAGE Stenotrophomonas maltophilia
3.5.1.2 41000
-
 gel filtration Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.2 L-glutamine + H2O Stenotrophomonas maltophilia
-
 L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O Stenotrophomonas maltophilia NYW-81
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Stenotrophomonas maltophilia
-
-
-
3.5.1.2 Stenotrophomonas maltophilia NYW-81
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2 native enzyme 250fold from strain NYW-81 to homogeneity by two steps of anion exchange chromatography, hydrophobic interaction and hydroxylapatite chromatography, and gel filtration Stenotrophomonas maltophilia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.2 additional information strain NYW-81, Koji medium, effects of carbon and nitrogen sources for cell growth on the production of enzyme, overview Stenotrophomonas maltophilia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.2 325
-
 purified enzyme Stenotrophomonas maltophilia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 D-asparagine + H2O 67% of the activity with L-glutamine Stenotrophomonas maltophilia D-aspartate + NH3
-
 ?
3.5.1.2 D-asparagine + H2O 67% of the activity with L-glutamine Stenotrophomonas maltophilia NYW-81 D-aspartate + NH3
-
 ?
3.5.1.2 D-glutamine + H2O 75% of the activity with L-glutamine Stenotrophomonas maltophilia D-glutamate + NH3
-
 ?
3.5.1.2 D-glutamine + H2O 75% of the activity with L-glutamine Stenotrophomonas maltophilia NYW-81 D-glutamate + NH3
-
 ?
3.5.1.2 L-asparagine + H2O 74% of the activity with L-glutamine Stenotrophomonas maltophilia L-aspartate + NH3
-
 ?
3.5.1.2 L-asparagine + H2O 74% of the activity with L-glutamine Stenotrophomonas maltophilia NYW-81 L-aspartate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O
-
 Stenotrophomonas maltophilia L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O best substrate Stenotrophomonas maltophilia L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O
-
 Stenotrophomonas maltophilia NYW-81 L-glutamate + NH3
-
 ?
3.5.1.2 L-glutamine + H2O best substrate Stenotrophomonas maltophilia NYW-81 L-glutamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.2 monomer 1 * 36000, SDS-PAGE Stenotrophomonas maltophilia

Synonyms

EC Number Synonyms Comment Organism
3.5.1.2 salt-tolerant glutaminase
-
 Stenotrophomonas maltophilia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.2 60
-
-
 Stenotrophomonas maltophilia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.2 60
-
 10 min, 70% remaining activity, purified enzyme Stenotrophomonas maltophilia
3.5.1.2 70
-
 10 min, 50% remaining activity, purified enzyme Stenotrophomonas maltophilia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.2 9
-
-
 Stenotrophomonas maltophilia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.2 5 10 most stable at pH 8.0, 60-80% of maximal activity at pH 5.0-10.0, purified enzyme Stenotrophomonas maltophilia