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Literature summary extracted from

  • Yoshimune, K.; Ninomiya, Y.; Wakayama, M.; Moriguchi, M.
    Molecular chaperones facilitate the soluble expression of N-acyl-D-amino acid amidohydrolases in Escherichia coli (2004), J. Ind. Microbiol. Biotechnol., 31, 421-426.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.81 three isozymes D-ANase, D-AGase, and D-AAase, soluble expression of the enzyme in Escherichia coli facilitated by molecular chaperones, overview Achromobacter xylosoxidans
3.5.1.82 overexpression of active D-AGase in Escherichia coli strain JM109 or strain BL21 partially in inclusion bodies, co-expression of chaperones GroELS, DnaKJE, and trigger factor TF Achromobacter xylosoxidans

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.81 Achromobacter xylosoxidans
-
 three isozymes D-ANase, D-AGase, and D-AAase
-
3.5.1.81 Achromobacter xylosoxidans A-6
-
 three isozymes D-ANase, D-AGase, and D-AAase
-
3.5.1.82 Achromobacter xylosoxidans
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.82 38.1
-
 soluble recombinant enzyme from expression without chaperones and trigger factor Achromobacter xylosoxidans
3.5.1.82 95.8
-
 soluble recombinant enzyme from co-expression with chaperones and trigger factor Achromobacter xylosoxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.81 additional information isozyme D-ANase is specific for N-acyl derivatives of neutral amino acids, while isozymes D-AAase and D-AGase are specific for N-acyl-D-aspartate and N-acyl-D-glutamate, respectively Achromobacter xylosoxidans ?
-
 ?
3.5.1.81 additional information isozyme D-ANase is specific for N-acyl derivatives of neutral amino acids, while isozymes D-AAase and D-AGase are specific for N-acyl-D-aspartate and N-acyl-D-glutamate, respectively Achromobacter xylosoxidans A-6 ?
-
 ?
3.5.1.81 N-acetyl-D-aspartate + H2O substrate of isozyme D-AAase Achromobacter xylosoxidans acetate + D-aspartate
-
 ?
3.5.1.81 N-acetyl-D-aspartate + H2O substrate of isozyme D-AAase Achromobacter xylosoxidans A-6 acetate + D-aspartate
-
 ?
3.5.1.81 N-acetyl-D-glutamate + H2O substrate of isozyme D-AGase Achromobacter xylosoxidans acetate + D-glutamate
-
 ?
3.5.1.81 N-acetyl-D-glutamate + H2O substrate of isozyme D-AGase Achromobacter xylosoxidans A-6 acetate + D-glutamate
-
 ?
3.5.1.82 additional information the enzyme is specific for N-acyl-D-glutamate Achromobacter xylosoxidans ?
-
 ?
3.5.1.82 additional information the enzyme is specific for N-acyl-D-glutamate Achromobacter xylosoxidans A-6 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.81 D-AAase
-
 Achromobacter xylosoxidans
3.5.1.81 D-AGase
-
 Achromobacter xylosoxidans
3.5.1.81 D-ANase
-
 Achromobacter xylosoxidans
3.5.1.81 N-Acyl-D-amino acid amidohydrolase
-
 Achromobacter xylosoxidans
3.5.1.82 D-AGase
-
 Achromobacter xylosoxidans
3.5.1.82 N-acyl-D-glutamate amidohydrolase
-
 Achromobacter xylosoxidans