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Literature summary extracted from

  • Gonzalez-Cuevas, J.; Bueno-Topete, M.; Armendariz-Borunda, J.
    Urokinase plasminogen activator stimulates function of active forms of stromelysin and gelatinases (MMP-2 and MMP-9) in cirrhotic tissue (2006), J. Gastroenterol. Hepatol., 21, 1544-1554.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.24.24 medicine endogenous urokinase plasminogen activator from cultured hepatic stellate cells significantly induces the active forms of enzyme and matrix metalloproteinase MMP-9 in cirrhotic tissue slices. Transfection of hepatic stellate cells with urokinase plasminogen activator gene results in overactivation of enzyme and matrix metalloproteinases MMP-3 and MMP-9 Rattus norvegicus
3.4.24.35 medicine endogenous urokinase plasminogen activator from cultured hepatic stellate cells significantly induces the active forms of enzyme and matrix metalloproteinase MMP-2 in cirrhotic tissue slices. Transfection of hepatic stellate cells with urokinase plasminogen activator gene results in overactivation of enzyme and matrix metalloproteinases MMP-3 and MMP-2 Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.73 functional overexpression of the enzyme in hepatic stellate cells, using an adenoviral vector system Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.73 additional information functional overexpression of the enzyme in hepatic stellate cells, using an adenoviral vector system, produces downregulation of pro-fibrogenic genes involved in liver fibrosis, and results in a highly specific decrease of TGF-beta expression, and at the same time of genes such as PAI-1, TIMP-1, and collagen type I, which respond to intracellular signals generated by TGF-beta and are involved in perpetuation of fibrogenesis, it also results in upregulation of MMP-3, MMP-2 and MMP-9 activities, overview Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.73 penicilloic acid
-
 Homo sapiens
3.4.21.73 plasminogen activator inhibitor-1
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.73 54000
-
 x * 54000 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.73 plasminogen + H2O Homo sapiens
-
 plasmin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.73 Homo sapiens
-
-
-
3.4.24.17 Rattus norvegicus
-
-
-
3.4.24.24 Rattus norvegicus
-
-
-
3.4.24.35 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.73 hepatic stellate cell high enzyme level Homo sapiens
-
3.4.21.73 HSC 180 cell a hepatic stellate cell line Homo sapiens
-
3.4.21.73 liver
-
 Homo sapiens
-
3.4.24.17 liver
-
 Rattus norvegicus
-
3.4.24.24 liver cirrhotic liver Rattus norvegicus
-
3.4.24.35 liver cirrhotic liver Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.73 plasminogen + H2O
-
 Homo sapiens plasmin + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.73 ? x * 54000 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.73 uPA
-
 Homo sapiens
3.4.21.73 Urokinase plasminogen activator
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.73 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.73 8.3
-
 assay at Homo sapiens