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Literature summary extracted from

  • Roux, C.; Salmon, L.; Verchere-Beaur, C.
    Preliminary studies on the inhibition of D-sorbitol-6-phosphate 2-dehydrogenase from Escherichia coli with substrate analogues (2006), J. Enzyme Inhib. Med. Chem., 21, 187-192.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.140 gene Ecs6pdh, overexpression in strain BL21 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.140 5-phospho-D-arabinonate substrate analogue inhibitor, IC50: 0.048 mM Escherichia coli
1.1.1.140 5-phospho-D-arabinonohydroxamic acid substrate analogue inhibitor, IC50: 0.040 mM Escherichia coli
1.1.1.140 D-mannose 6-phosphate potent inhibitor, IC50: 0.0075 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.140 0.025
-
 NADH pH 7.5, 25°C, recombinant enzyme Escherichia coli
1.1.1.140 0.568
-
 D-fructose 6-phosphate pH 7.5, 25°C, recombinant enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.140 D-fructose 6-phosphate + NADH Escherichia coli
-
 D-sorbitol 6-phosphate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.140 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.140 recombinant enzyme from strain BL21 by ammonium sulfate fractionation and gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.140 D-fructose 6-phosphate + NADH
-
 Escherichia coli D-sorbitol 6-phosphate + NAD+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.140 D-sorbitol-6-phosphate 2-dehydrogenase
-
 Escherichia coli
1.1.1.140 S6PDH
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.140 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.140 7.5
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.140 NAD+
-
 Escherichia coli
1.1.1.140 NADH
-
 Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.140 0.0075
-
 potent inhibitor, IC50: 0.0075 mM Escherichia coli D-mannose 6-phosphate
1.1.1.140 0.04
-
 substrate analogue inhibitor, IC50: 0.040 mM Escherichia coli 5-phospho-D-arabinonohydroxamic acid
1.1.1.140 0.048
-
 substrate analogue inhibitor, IC50: 0.048 mM Escherichia coli 5-phospho-D-arabinonate