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Literature summary extracted from

  • Kotzia, G.A.; Labrou, N.E.
    Cloning, expression and characterisation of Erwinia carotovora L-asparaginase (2005), J. Biotechnol., 119, 309-323.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.1 medicine the enzyme is used as therapeutic agent in the treatment of acute childhood lymphoblastic leukemia Pectobacterium carotovorum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expression in Escherichia coli Pectobacterium carotovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 additional information
-
 additional information thermodynamic analysis, kinetic study and molecular modelling Pectobacterium carotovorum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Pectobacterium carotovorum
-
 L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O Pectobacterium carotovorum NCYC 1526
-
 L-aspartate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Pectobacterium carotovorum
-
-
-
3.5.1.1 Pectobacterium carotovorum NCYC 1526
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 recombinant enzyme from Escherichia coli to homogeneity by cation exchange and L-asparagine affinity chromatography Pectobacterium carotovorum

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.1 L-asparagine + H2O = L-aspartate + NH3 kinetic model, substrate specificity, and catalytic mechanism, active site residues are Asp115 and Glu82, the structural transition of the enzyme is rate-limiting in the reaction, entropy changes are the main determinants for the substrate specificity Pectobacterium carotovorum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Pectobacterium carotovorum L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Pectobacterium carotovorum NCYC 1526 L-aspartate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 ErA
-
 Pectobacterium carotovorum
3.5.1.1 L-asparaginase
-
 Pectobacterium carotovorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 additional information
-
-
 Pectobacterium carotovorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 additional information
-
-
 Pectobacterium carotovorum