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Literature summary extracted from

  • Garcia-Conesa, M.T.; Crepin, V.F.; Goldson, A.J.; Williamson, G.; Cummings, N.J.; Connerton, I.F.; Faulds, C.B.; Kroon, P.A.
    The feruloyl esterase system of Talaromyces stipitatus: production of three discrete feruloyl esterases, including a novel enzyme, TsFaeC, with a broad substrate specificity (2004), J. Biotechnol., 108, 227-241.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 type C enzyme Talaromyces stipitatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Talaromyces stipitatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 35000
-
 x * 35000, type A and B enzymes, SDS-PAGE Talaromyces stipitatus
3.1.1.73 55340
-
 x * 55340, type C enzyme, calculated from the deduced amino acid sequence, x * 66000, smeared band on SDS-PAGE Talaromyces stipitatus
3.1.1.73 66000
-
 x * 55340, type C enzyme, calculated from the deduced amino acid sequence, x * 66000, smeared band on SDS-PAGE Talaromyces stipitatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 feruloyl polysaccharide + H2O Talaromyces stipitatus involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O Talaromyces stipitatus CBS 375.48 involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components ferulic acid + ?
-
 ?
3.1.1.73 sugar beat pulp + H2O Talaromyces stipitatus
-
 ferulic acid + ?
-
 ?
3.1.1.73 sugar beat pulp + H2O Talaromyces stipitatus CBS 375.48
-
 ferulic acid + ?
-
 ?
3.1.1.73 wheat bran + H2O Talaromyces stipitatus
-
 ferulic acid + ?
-
 ?
3.1.1.73 wheat bran + H2O Talaromyces stipitatus CBS 375.48
-
 ferulic acid + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Talaromyces stipitatus
-
 feruloyl ersterasea type A and B
-
3.1.1.73 Talaromyces stipitatus Q70Y21 feruloyl ersterasea type C
-
3.1.1.73 Talaromyces stipitatus CBS 375.48
-
 feruloyl ersterasea type A and B
-
3.1.1.73 Talaromyces stipitatus CBS 375.48 Q70Y21 feruloyl ersterasea type C
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.73 glycoprotein difference between theoretical and detected molecular mass indicates glycosylation Talaromyces stipitatus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 type A and B enzyme Talaromyces stipitatus
3.1.1.73 type C enzyme Talaromyces stipitatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Talaromyces stipitatus ferulic acid + ?
-
 ?
3.1.1.73 feruloyl polysaccharide + H2O involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components Talaromyces stipitatus CBS 375.48 ferulic acid + ?
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Talaromyces stipitatus caffeic acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O very low activity of type A enzyme Talaromyces stipitatus caffeic acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O very low activity of type A enzyme Talaromyces stipitatus CBS 375.48 caffeic acid + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Talaromyces stipitatus CBS 375.48 caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Talaromyces stipitatus ferulic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O low activity of type B enzyme Talaromyces stipitatus ferulic acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Talaromyces stipitatus p-coumaric acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O very low activity of type A enzyme Talaromyces stipitatus p-coumaric acid + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Talaromyces stipitatus sinapic acid + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Talaromyces stipitatus sinapic acid + methanol no activity of type B enzyme ?
3.1.1.73 methyl sinapinate + H2O
-
 Talaromyces stipitatus CBS 375.48 sinapic acid + methanol no activity of type B enzyme ?
3.1.1.73 methyl sinapinate + H2O
-
 Talaromyces stipitatus CBS 375.48 sinapic acid + methanol
-
 ?
3.1.1.73 O-[5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl]-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + H2O high activity of type A enzyme, moderate or low activity of type C and B enzymes Talaromyces stipitatus ferulic acid + ?
-
 ?
3.1.1.73 O-[5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl]-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + H2O low activity of type C enzyme Talaromyces stipitatus ferulic acid + ?
-
 ?
3.1.1.73 sugar beat pulp + H2O
-
 Talaromyces stipitatus ferulic acid + ?
-
 ?
3.1.1.73 sugar beat pulp + H2O
-
 Talaromyces stipitatus CBS 375.48 ferulic acid + ?
-
 ?
3.1.1.73 wheat bran + H2O
-
 Talaromyces stipitatus ferulic acid + ?
-
 ?
3.1.1.73 wheat bran + H2O
-
 Talaromyces stipitatus CBS 375.48 ferulic acid + ?
-
 ?
3.1.1.73 [2-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1,5)-L-arabinofuranose + H2O
-
 Talaromyces stipitatus ferulic acid + ?
-
 ?
3.1.1.73 [2-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1,5)-L-arabinofuranose + H2O no activity of type A enzyme Talaromyces stipitatus ferulic acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 35000, type A and B enzymes, SDS-PAGE Talaromyces stipitatus
3.1.1.73 ? x * 55340, type C enzyme, calculated from the deduced amino acid sequence, x * 66000, smeared band on SDS-PAGE Talaromyces stipitatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 60
-
 type C enzyme Talaromyces stipitatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 70
-
 20% of activity Talaromyces stipitatus
3.1.1.73 80
-
 4% of activity Talaromyces stipitatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 40
-
 unstable on incubation above 40°C Talaromyces stipitatus
3.1.1.73 60
-
 almost complete loss of activity within 10 min Talaromyces stipitatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 6 7 type C enzyme Talaromyces stipitatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 2.6
-
 34% of activity Talaromyces stipitatus
3.1.1.73 4
-
 50% of activity Talaromyces stipitatus
3.1.1.73 5.6
-
 95% of activity Talaromyces stipitatus
3.1.1.73 7.7
-
 88% of activity Talaromyces stipitatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 Talaromyces stipitatus type A enzyme, isoelectric focusing
-
 3.5
3.1.1.73 Talaromyces stipitatus type C enzyme, isoelectric focusing
-
 4.6
3.1.1.73 Talaromyces stipitatus type B enzyme, isoelectric focusing
-
 5.3