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Literature summary extracted from

  • Higashi, N.; Fukada, H.; Ishikawa, K.
    Kinetic study of thermostable L-threonine dehydrogenase from an archaeon Pyrococcus horikoshii (2005), J. Biosci. Bioeng., 99, 175-180.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.103 orf PH0655, gene TDH, overexpression in Escherichia coli strain BL21(DE3) Pyrococcus horikoshii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.103 EDTA complete inhibition at 50 mM Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.103 additional information
-
 additional information kinetics Pyrococcus horikoshii
1.1.1.103 0.01
-
 NAD+ pH 7.5, 65°C Pyrococcus horikoshii
1.1.1.103 0.013
-
 L-threonine pH 7.5, 65°C Pyrococcus horikoshii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.103 Ca2+ 0.54 mol Ca2+ per mol of enzyme subunit Pyrococcus horikoshii
1.1.1.103 Cu2+ 0.29 mol Ca2+ per mol of enzyme subunit Pyrococcus horikoshii
1.1.1.103 Mg2+ 0.2 mol Ca2+ per mol of enzyme subunit Pyrococcus horikoshii
1.1.1.103 Zn2+ required, 1.22 mol Zn2+ per mol of enzyme subunit Pyrococcus horikoshii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.103 37742
-
 2 * 36000-38000, recombinant enzyme, SDS-PAGE, 2 * 37742, sequence calculation Pyrococcus horikoshii
1.1.1.103 76000
-
 recombinant enzyme, gel filtration Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.103 L-threonine + NAD+ Pyrococcus horikoshii
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.103 Pyrococcus horikoshii
-
 orf PH0655, gene TDH
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.103 recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 85°C for 30 min, centrifugation, ion exchange chromatography, and gel filtration Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.103 L-threonine + NAD+
-
 Pyrococcus horikoshii (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.103 dimer 2 * 36000-38000, recombinant enzyme, SDS-PAGE, 2 * 37742, sequence calculation Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.103 L-threonine dehydrogenase
-
 Pyrococcus horikoshii
1.1.1.103 TDH
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.103 65
-
 assay at Pyrococcus horikoshii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.103 50 106
-
 Pyrococcus horikoshii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.103 108
-
 2-step thermal inactivation Pyrococcus horikoshii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.103 7.2
-
 NAD+ pH 7.5, 65°C Pyrococcus horikoshii
1.1.1.103 7.2
-
 L-threonine pH 7.5, 65°C Pyrococcus horikoshii
1.1.1.103 551.7
-
 NAD+ pH 7.5, 65°C Pyrococcus horikoshii
1.1.1.103 551.7
-
 L-threonine pH 7.5, 65°C Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.103 7.5
-
 assay at Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.103 NAD+ dependent on Pyrococcus horikoshii