Any feedback?
Literature summary extracted from
- Kinetic and spectroscopic characterization of the E134A- and E134D-altered dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from Haemophilus influenzae (2006), J. Biol. Inorg. Chem., 11, 206-216.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.18 | expressed in Escherichia coli | Haemophilus influenzae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.1.18 | E134A | Glu134 replaced by alanine | Haemophilus influenzae |
| 3.5.1.18 | E134D | Glu134 replaced by aspartate | Haemophilus influenzae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.18 | 0.65 | - |
N-succinyl-LL-diaminopimelate | mutant enzyme E134D at pH 7.5 | Haemophilus influenzae |  |
| 3.5.1.18 | 0.73 | - |
N-succinyl-LL-diaminopimelate | wild type enzyme at pH 7.5 | Haemophilus influenzae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.18 | 41500 | - |
SDS-PAGE | Haemophilus influenzae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.18 | Haemophilus influenzae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.18 | fast-flow Q-sepharose anionexchange column chromatography | Haemophilus influenzae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.18 | 0.17 | - |
mutant enzyme E134D at pH 7.5 | Haemophilus influenzae |
| 3.5.1.18 | 180 | - |
wild type enzyme at pH 7.5 | Haemophilus influenzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.18 | N-succinyl-LL-diaminopimelate + H2O | - |
Haemophilus influenzae | ? | - |
? | |
| 3.5.1.18 | N-succinyl-LL-diaminopimelic acid + H2O | - |
Haemophilus influenzae | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.18 | DapE | - |
Haemophilus influenzae |
| 3.5.1.18 | dapE-encoded N-succinyl-LL-diaminopimelic acid desuccinylase | - |
Haemophilus influenzae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.18 | 0.13 | - |
N-succinyl-LL-diaminopimelate | mutant enzyme E134D at pH 7.5 | Haemophilus influenzae | |
| 3.5.1.18 | 140 | - |
N-succinyl-LL-diaminopimelate | wild type enzyme at pH 7.5 | Haemophilus influenzae | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.18 | 7.5 | 8.5 | - |
Haemophilus influenzae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.18 | 6 | 9 | activity below pH 6.0 was not observed | Haemophilus influenzae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.18 | 6 | 9 | activity below pH 6.0 was not observed | Haemophilus influenzae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
