Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Anderson, P.J.; Kokame, K.; Sadler, J.E.
    Zinc and calcium ions cooperatively modulate ADAMTS13 activity (2006), J. Biol. Chem., 281, 850-857.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.87
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.87 CoCl2 an increasing concentration of CoCl2 inhibits ADAMTS13 activity Homo sapiens
3.4.24.87 MnSO4 an increasing concentration of MnSO4 inhibits ADAMTS13 activity Homo sapiens
3.4.24.87 additional information increasing concentrations of BaCl2 (up to 5 mM) have little effect on the activity of ADAMTS13 at pH 7.4, Mg(SO4) and Cu(SO4) have little effect on the activity of ADAMTS13 at pH 7.4 Homo sapiens
3.4.24.87 NiSO4 an increasing concentration of NiSO4 inhibits ADAMTS13 activity Homo sapiens
3.4.24.87 Zn2+ ADAMTS13 activity is undetectable at concentrations of zinc ions above 3 mM Zn2+ Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.87 additional information
-
 additional information Km: 0.0032 mM for FRETS-VWF73, a fluorescent synthetic peptide, corresponding to residues Asp1596-Arg1668 of von Willebrand factor domain A2 and containing the Tyr1605-Met1606 bond cleaved by ADAMTS13 Homo sapiens
3.4.24.87 0.0032
-
 FRETS-von Willebrand factor 73 apparent value, pH 7.4 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.87 Ba2+ barium ions stimulate ADAMTS13 activity in citrated plasma but not in citrate-free plasma, cleavage of von Willebrand factor by recombinant ADAMTS13 is activated up to 200fold by barium ions Homo sapiens
3.4.24.87 Ca2+ cooperative role for Ca2+ and Zn2+ in supporting ADAMTS13 activity Homo sapiens
3.4.24.87 Ca2+ in citrate-anticoagulated plasma activity is enhanced about 3fold by calcium ions (up to 5 mM), cleavage of von Willebrand factor by recombinant ADAMTS13 is activated up to 200fold by calcium ions Homo sapiens
3.4.24.87 Zn2+ cooperative role for Ca2+ and Zn2+ in supporting ADAMTS13 activity Homo sapiens
3.4.24.87 Zn2+ in citrate-anticoagulated plasma activity is enhanced about 2fold by zinc ions (1 mM), cleavage of von Willebrand factor by recombinant ADAMTS13 is activated up to 200fold by zinc ions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.87 von Willebrand factor + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.87 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.87 blood plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.87 FRETS-von Willebrand factor 73 + H2O
-
 Homo sapiens ?
-
 ?
3.4.24.87 von Willebrand factor + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.87 ADAMTS13
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.87 additional information
-
 additional information kcat: 0.83 per min for FRETS-VWF73, a fluorescent synthetic peptide, corresponding to residues Asp1596-Arg1668 of von Willebrand factor domain A2 and containing the Tyr1605-Met1606 bond cleaved by ADAMTS13 Homo sapiens