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Literature summary extracted from

  • Kono, M.; Dreier, J.L.; Ellis, J.M.; Allende, M.L.; Kalkofen, D.N.; Sanders, K.M.; Bielawski, J.; Bielawska, A.; Hannun, Y.A.; Proia, R.L.
    Neutral ceramidase encoded by the Asah2 gene is essential for the intestinal degradation of sphingolipids (2006), J. Biol. Chem., 281, 7324-7331.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.23 gene Asah2, DNA and amino acid sequence determination and analysis, expression in ES cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.23 additional information construction of disruption mutant of Asah2 by using a targeted ES clone to produce chimeras and subsequently heterozygous mice, interbreeding of the Asah2 heterozygous mice yielded wild-type, heterozygous, and homozygous mutant null offspring, Asah2 null mice are deficient in the intestinal degradation of ceramide, Asah2 null mice have a normal life span and do not show obvious abnormalities or major alterations in total ceramide levels in tissues Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 brush border
-
Mus musculus 5903
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.23 N-acyl-sphingosine + H2O Mus musculus the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the cellular levels of bioactive sphingolipid metabolites, ceramide, sphingosine, and sphingosine 1-phosphate, in the intestinal tract a carboxylate + sphingosine
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Mus musculus
-
gene Asah2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 brain
-
Mus musculus
-
3.5.1.23 cecum
-
Mus musculus
-
3.5.1.23 colon
-
Mus musculus
-
3.5.1.23 duodenum
-
Mus musculus
-
3.5.1.23 ileum
-
Mus musculus
-
3.5.1.23 jejunum
-
Mus musculus
-
3.5.1.23 kidney
-
Mus musculus
-
3.5.1.23 liver
-
Mus musculus
-
3.5.1.23 additional information tissue expression pattern, oveview Mus musculus
-
3.5.1.23 small intestine high enzyme expresion level along the brush border Mus musculus
-
3.5.1.23 stomach
-
Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.23 additional information
-
determination of sphingolipid levels by mass spectrometry Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O
-
Mus musculus 4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide
-
?
3.5.1.23 N-acyl-sphingosine + H2O
-
Mus musculus a carboxylate + sphingosine
-
?
3.5.1.23 N-acyl-sphingosine + H2O the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the cellular levels of bioactive sphingolipid metabolites, ceramide, sphingosine, and sphingosine 1-phosphate, in the intestinal tract Mus musculus a carboxylate + sphingosine
-
?
3.5.1.23 palmitoyl-D-[erythro-9,10]sphingosine + H2O
-
Mus musculus palmitate + D-erythro-sphingosine
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 neutral ceramidase
-
Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.23 37
-
assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 7.5
-
assay at Mus musculus