EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.23 | gene Asah2, DNA and amino acid sequence determination and analysis, expression in ES cells | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.23 | additional information | construction of disruption mutant of Asah2 by using a targeted ES clone to produce chimeras and subsequently heterozygous mice, interbreeding of the Asah2 heterozygous mice yielded wild-type, heterozygous, and homozygous mutant null offspring, Asah2 null mice are deficient in the intestinal degradation of ceramide, Asah2 null mice have a normal life span and do not show obvious abnormalities or major alterations in total ceramide levels in tissues | Mus musculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.23 | brush border | - |
Mus musculus | 5903 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.23 | N-acyl-sphingosine + H2O | Mus musculus | the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the cellular levels of bioactive sphingolipid metabolites, ceramide, sphingosine, and sphingosine 1-phosphate, in the intestinal tract | a carboxylate + sphingosine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.23 | Mus musculus | - |
gene Asah2 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.1.23 | brain | - |
Mus musculus | - |
3.5.1.23 | cecum | - |
Mus musculus | - |
3.5.1.23 | colon | - |
Mus musculus | - |
3.5.1.23 | duodenum | - |
Mus musculus | - |
3.5.1.23 | ileum | - |
Mus musculus | - |
3.5.1.23 | jejunum | - |
Mus musculus | - |
3.5.1.23 | kidney | - |
Mus musculus | - |
3.5.1.23 | liver | - |
Mus musculus | - |
3.5.1.23 | additional information | tissue expression pattern, oveview | Mus musculus | - |
3.5.1.23 | small intestine | high enzyme expresion level along the brush border | Mus musculus | - |
3.5.1.23 | stomach | - |
Mus musculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.23 | additional information | - |
determination of sphingolipid levels by mass spectrometry | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.23 | 4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O | - |
Mus musculus | 4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide | - |
? | |
3.5.1.23 | N-acyl-sphingosine + H2O | - |
Mus musculus | a carboxylate + sphingosine | - |
? | |
3.5.1.23 | N-acyl-sphingosine + H2O | the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the cellular levels of bioactive sphingolipid metabolites, ceramide, sphingosine, and sphingosine 1-phosphate, in the intestinal tract | Mus musculus | a carboxylate + sphingosine | - |
? | |
3.5.1.23 | palmitoyl-D-[erythro-9,10]sphingosine + H2O | - |
Mus musculus | palmitate + D-erythro-sphingosine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.23 | neutral ceramidase | - |
Mus musculus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.23 | 37 | - |
assay at | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.23 | 7.5 | - |
assay at | Mus musculus |