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Literature summary extracted from

  • Stafforini, D.M.; Sheller, J.R.; Blackwell, T.S.; Sapirstein, A.; Yull, F.E.; McIntyre, T.M.; Bonventre, J.V.; Prescott, S.M.; Roberts, L.J.2nd.
    Release of free F2-isoprostanes from esterified phospholipids is catalyzed by intracellular and plasma platelet-activating factor acetylhydrolases (2006), J. Biol. Chem., 281, 4616-4623.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.47 expressed as FLAG-tag fusion protein in COS-7 cells, construction of transgenic mice Homo sapiens
3.1.1.47 expression in COS7 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.47 0.0000045
-
 F2-isoprostane-phosphocholine purified recombinant plasma enzyme, 37°C Homo sapiens
3.1.1.47 0.000057
-
 F2-isoprostane-phosphocholine purified recombinant PAF-acetylhydrolase, 37°C Homo sapiens
3.1.1.47 0.0052
-
 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine purified recombinant plasma enzyme, 37°C Homo sapiens
3.1.1.47 0.0135
-
 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine purified recombinant PAF-acetylhydrolase, 37°C Homo sapiens
3.1.1.47 0.0193
-
 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine purified recombinant plasma enzyme, 37°C Homo sapiens
3.1.1.47 0.0431
-
 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine purified recombinant PAF-acetylhydrolase, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.47 extracellular
-
 Homo sapiens
-
-
3.1.1.47 intracellular
-
 Homo sapiens 5622
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.47 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O Homo sapiens regulation of platelet-activating factor 1-alkyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 F2-isoprostane phospholipid + H2O Homo sapiens plays key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo, involved in oxidative stress response F2-isoprostane + phospholipid
-
 ?
3.1.1.47 additional information Homo sapiens PAF acetylhydrolases play key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.47 Homo sapiens
-
-
-
3.1.1.47 Homo sapiens
-
 Japanese patients with partial or complete plasma enzyme deficiency and control subjects
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.47 recombinant protein using FLAG-tag Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.47 blood plasma
-
 Homo sapiens
-
3.1.1.47 blood plasma enzyme associated with low-density lipoprotein and high-density lipoprotein Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.47 4.2
-
 purifed enzyme using 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine as substrate Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.47 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O regulation of platelet-activating factor Homo sapiens 1-alkyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine + H2O
-
 Homo sapiens 5-oxopentanoate + 1-palmitoyl-sn-glycero-3-phosphocholine
-
 ?
3.1.1.47 F2-isoprostane phospholipid + H2O plays key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo, involved in oxidative stress response Homo sapiens F2-isoprostane + phospholipid
-
 ?
3.1.1.47 F2-isoprostane-phosphocholine + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.47 additional information PAF acetylhydrolases play key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.47 PAF-acetylhydrolase II
-
 Homo sapiens
3.1.1.47 platelet-activating factor acetylhydrolase
-
 Homo sapiens