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Literature summary extracted from

  • Plummer, L.J.; Hildebrandt, E.R.; Porter, S.B.; Rogers, V.A.; McCracken, J.; Schmidt, W.K.
    Mutational analysis of the ras converting enzyme reveals a requirement for glutamate and histidine residues (2006), J. Biol. Chem., 281, 4596-4605.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.4.99.B1 C251A wild-type in function Saccharomyces cerevisiae
3.4.99.B1 E156A activity only when overexpressed Saccharomyces cerevisiae
3.4.99.B1 E156D marginal activity, overexpression significantly boosts the activity Saccharomyces cerevisiae
3.4.99.B1 E156Q no activity Saccharomyces cerevisiae
3.4.99.B1 E157A diminished but significant activity Saccharomyces cerevisiae
3.4.99.B1 F190A diminished but significant activity Saccharomyces cerevisiae
3.4.99.B1 H194A invariably inactive Saccharomyces cerevisiae
3.4.99.B1 H196A wild-type in function Saccharomyces cerevisiae
3.4.99.B1 H197A wild-type in function Saccharomyces cerevisiae
3.4.99.B1 H248A activity only when overexpressed Saccharomyces cerevisiae
3.4.99.B1 additional information strain yWS163, that lacks the a-factor encoding genes and the CAAX protease genes Saccharomyces cerevisiae
3.4.99.B1 N252A diminished but significant activity Saccharomyces cerevisiae
3.4.99.B1 Y160A diminished but significant activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.99.B1 0.00144
-
 a-factor derived synthetic farnesylated pentadecapeptide mutant H197A Saccharomyces cerevisiae
3.4.99.B1 0.00172
-
 a-factor derived synthetic farnesylated pentadecapeptide wild type Saccharomyces cerevisiae
3.4.99.B1 0.00234
-
 a-factor derived synthetic farnesylated pentadecapeptide mutant C251A Saccharomyces cerevisiae
3.4.99.B1 0.00326
-
 a-factor derived synthetic farnesylated pentadecapeptide mutant H196A Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.99.B1 membrane
-
 Homo sapiens 16020
-
3.4.99.B1 membrane
-
 Saccharomyces cerevisiae 16020
-
3.4.99.B1 membrane
-
 Arabidopsis thaliana 16020
-
3.4.99.B1 membrane
-
 Caenorhabditis elegans 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.99.B1 Arabidopsis thaliana
-
-
-
3.4.99.B1 Caenorhabditis elegans
-
-
-
3.4.99.B1 Drosophila melanogaster
-
-
-
3.4.99.B1 Homo sapiens
-
-
-
3.4.99.B1 Saccharomyces cerevisiae
-
 strain RC757, strain SM1068, strain SM3614, strain STY50
-
3.4.99.B1 Schizosaccharomyces pombe
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.99.B1 a-factor derived synthetic farnesylated pentadecapeptide + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.99.B1 CALM + H2O
-
 Homo sapiens ?
-
 ?
3.4.99.B1 CALM + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.99.B1 CALQ + H2O
-
 Homo sapiens ?
-
 ?
3.4.99.B1 CALQ + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.99.B1 CAMQ + H2O
-
 Homo sapiens ?
-
 ?
3.4.99.B1 CAMQ + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.99.B1 CASQ + H2O no cleavage when RCE1 is expressed from low copy plasmids or is overexpressed Arabidopsis thaliana ?
-
 ?
3.4.99.B1 CASQ + H2O no cleavage when RCE1 is expressed from low copy plasmids or is overexpressed Caenorhabditis elegans ?
-
 ?
3.4.99.B1 CASQ + H2O no cleavage when RCE1 is expressed from low copy plasmids, but overexpressed RCE1 appears to have marginal activity towards CASQ Saccharomyces cerevisiae ?
-
 ?
3.4.99.B1 CSVM + H2O
-
 Homo sapiens ?
-
 ?
3.4.99.B1 CSVM + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.99.B1 CTLM + H2O
-
 Homo sapiens ?
-
 ?
3.4.99.B1 CTLM + H2O one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed Saccharomyces cerevisiae ?
-
 ?
3.4.99.B1 CTLM + H2O one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed Arabidopsis thaliana ?
-
 ?
3.4.99.B1 CTLM + H2O one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed Caenorhabditis elegans ?
-
 ?
3.4.99.B1 CTSM + H2O
-
 Homo sapiens ?
-
 ?
3.4.99.B1 CTSM + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.99.B1 CTVM + H2O
-
 Homo sapiens ?
-
 ?
3.4.99.B1 CTVM + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.4.99.B1 CVIA + H2O
-
 Homo sapiens ?
-
 ?
3.4.99.B1 CVIA + H2O one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed Saccharomyces cerevisiae ?
-
 ?
3.4.99.B1 CVIA + H2O one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed Arabidopsis thaliana ?
-
 ?
3.4.99.B1 CVIA + H2O one of the preferred CAAX motifs, when RCE1 is expressed from low copy plasmids or is overexpressed Caenorhabditis elegans ?
-
 ?
3.4.99.B1 additional information CTSQ motif poorly cleaved Homo sapiens ?
-
 ?
3.4.99.B1 additional information CTSQ motif poorly cleaved Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.99.B1 Ras-converting enzyme
-
 Drosophila melanogaster
3.4.99.B1 Ras-converting enzyme
-
 Homo sapiens
3.4.99.B1 Ras-converting enzyme
-
 Saccharomyces cerevisiae
3.4.99.B1 Ras-converting enzyme
-
 Arabidopsis thaliana
3.4.99.B1 Ras-converting enzyme
-
 Schizosaccharomyces pombe
3.4.99.B1 Ras-converting enzyme
-
 Caenorhabditis elegans
3.4.99.B1 RCE1
-
 Drosophila melanogaster
3.4.99.B1 RCE1
-
 Homo sapiens
3.4.99.B1 RCE1
-
 Saccharomyces cerevisiae
3.4.99.B1 RCE1
-
 Arabidopsis thaliana
3.4.99.B1 RCE1
-
 Schizosaccharomyces pombe
3.4.99.B1 RCE1
-
 Caenorhabditis elegans
3.4.99.B1 Rce1p
-
 Saccharomyces cerevisiae