EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.376 | overexpression in Escherichia coli | Azospirillum brasilense |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.376 | D168A | mutant shows no activity under standard assay conditions | Azospirillum brasilense |
1.1.1.376 | N172A | kinetic analysis shows that the N172A mutant decreases by 4 orders of magnitude in kcat/Km values, compared with the wild-type enzyme | Azospirillum brasilense |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.376 | 0.109 | - |
D-galactose | pH 9.0, 30°C, cofactor: NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 0.255 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 0.26 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NADP+, recombinant wild-type enzyme | Azospirillum brasilense | |
1.1.1.376 | 0.785 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NAD+, recombinant wild-type enzyme | Azospirillum brasilense | |
1.1.1.376 | 1.41 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 1.49 | - |
D-galactose | pH 9.0, 30°C, cofactor: NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 2 | 10 | D-xylose | pH 9.0, 30°C, cofactor: NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 3.95 | - |
D-talose | pH 9.0, 30°C, cofactor: NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 5.87 | - |
D-talose | pH 9.0, 30°C, cofactor: NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 28.9 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NADP+, mutant enzyme N172A | Azospirillum brasilense | |
1.1.1.376 | 42.5 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NAD+, mutant enzyme N172A | Azospirillum brasilense | |
1.1.1.376 | 72 | - |
D-xylose | pH 9.0, 30°C, cofactor: NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.376 | additional information | no significant increase in activity in the presence of MgCl2, MnCl2, ZnCl2, CoCl2, NiCl2, or CaCl2 at final concentrations of 1 mM | Azospirillum brasilense |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.376 | 33664 | - |
1 * 33664, calculated from sequence | Azospirillum brasilense |
1.1.1.376 | 39500 | - |
1 * 39500, SDS-PAGE | Azospirillum brasilense |
1.1.1.376 | 46400 | - |
gel filtration | Azospirillum brasilense |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.376 | L-arabinose + NADP+ | Azospirillum brasilense | first step of L-arabinose metabolism. The enzyme is involved in the metabolism of L-arabinose but not D-galactose | L-arabinono-1,4-lactone + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.376 | Azospirillum brasilense | Q53TZ2 | - |
- |
1.1.1.376 | Azospirillum brasilense DSM 1690 | Q53TZ2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.376 | - |
Azospirillum brasilense |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.376 | 0.042 | - |
pH 9.0, 30°C, L-arabinose + NAD+, mutant enzyme N172A | Azospirillum brasilense |
1.1.1.376 | 0.148 | - |
pH 9.0, 30°C, L-arabinose + NADP+, mutant enzyme N172A | Azospirillum brasilense |
1.1.1.376 | 1.7 | - |
pH 9.0, 30°C, D-talose + NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense |
1.1.1.376 | 5.3 | - |
pH 9.0, 30°C, D-xylose + NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense |
1.1.1.376 | 12.8 | - |
pH 9.0, 30°C, D-talose + NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense |
1.1.1.376 | 14.8 | - |
pH 9.0, 30°C, D-xylose + NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense |
1.1.1.376 | 14.9 | - |
pH 9.0, 30°C, L-arabinose + NAD+, recombinant wild-type enzyme | Azospirillum brasilense |
1.1.1.376 | 23.8 | - |
pH 9.0, 30°C, D-galactose + NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense |
1.1.1.376 | 25 | - |
pH 9.0, 30°C, L-arabinose + NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense |
1.1.1.376 | 32 | - |
pH 9.0, 30°C, L-arabinose + NADP+, recombinant wild-type enzyme | Azospirillum brasilense |
1.1.1.376 | 35.6 | - |
pH 9.0, 30°C, D-galactose + NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense |
1.1.1.376 | 44.9 | - |
pH 9.0, 30°C, L-arabinose + NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.376 | D-galactose + NAD+ | the enzyme shows high catalytic efficiency for both L-arabinose and D-galactose. The enzyme prefers NADP+ over NAD+ | Azospirillum brasilense | ? | - |
? | |
1.1.1.376 | D-galactose + NAD+ | the enzyme shows high catalytic efficiency for both L-arabinose and D-galactose. The enzyme prefers NADP+ over NAD+ | Azospirillum brasilense DSM 1690 | ? | - |
? | |
1.1.1.376 | D-galactose + NADP+ | the enzyme shows high catalytic efficiency for both L-arabinose and D-galactose. The enzyme prefers NADP+ over NAD+ | Azospirillum brasilense | ? | - |
? | |
1.1.1.376 | D-galactose + NADP+ | the enzyme shows high catalytic efficiency for both L-arabinose and D-galactose. The enzyme prefers NADP+ over NAD+ | Azospirillum brasilense DSM 1690 | ? | - |
? | |
1.1.1.376 | D-talose + NAD+ | low activity | Azospirillum brasilense | ? | - |
? | |
1.1.1.376 | D-talose + NADP+ | low activity | Azospirillum brasilense | ? | - |
? | |
1.1.1.376 | D-xylose + NAD+ | low activity | Azospirillum brasilense | ? | - |
? | |
1.1.1.376 | D-xylose + NAD+ | low activity | Azospirillum brasilense DSM 1690 | ? | - |
? | |
1.1.1.376 | D-xylose + NADP+ | low activity | Azospirillum brasilense | ? | - |
? | |
1.1.1.376 | D-xylose + NADP+ | low activity | Azospirillum brasilense DSM 1690 | ? | - |
? | |
1.1.1.376 | L-arabinose + NAD+ | the enzyme shows high catalytic efficiency for both L-arabinose and D-galactose. The enzyme prefers NADP+ over NAD+ | Azospirillum brasilense | L-arabinono-1,4-lactone + NADH + H+ | - |
? | |
1.1.1.376 | L-arabinose + NADP+ | first step of L-arabinose metabolism. The enzyme is involved in the metabolism of L-arabinose but not D-galactose | Azospirillum brasilense | L-arabinono-1,4-lactone + NADPH + H+ | - |
? | |
1.1.1.376 | L-arabinose + NADP+ | the enzyme shows high catalytic efficiency for both L-arabinose and D-galactose. The enzyme prefers NADP+ over NAD+ | Azospirillum brasilense | L-arabinono-1,4-lactone + NADPH + H+ | - |
? | |
1.1.1.376 | additional information | no activity with D-arabinose, D-glucose, D-ribose, L-xylose, L-mannose, L-lyxose, and D-fructose (less than 1% of the activity with L-arabinose) | Azospirillum brasilense | ? | - |
? | |
1.1.1.376 | additional information | no activity with D-arabinose, D-glucose, D-ribose, L-xylose, L-mannose, L-lyxose, and D-fructose (less than 1% of the activity with L-arabinose) | Azospirillum brasilense DSM 1690 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.376 | monomer | 1 * 39500, SDS-PAGE | Azospirillum brasilense |
1.1.1.376 | monomer | 1 * 33664, calculated from sequence | Azospirillum brasilense |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.376 | L-arabinose 1-dehydrogenase | ambiguous | Azospirillum brasilense |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.376 | 30 | - |
assay at | Azospirillum brasilense |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.376 | 0.1 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NAD+, mutant enzyme N172A | Azospirillum brasilense | |
1.1.1.376 | 0.3 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NADP+, mutant enzyme N172A | Azospirillum brasilense | |
1.1.1.376 | 1.04 | - |
D-talose | pH 9.0, 30°C, cofactor: NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 4 | - |
D-xylose | pH 9.0, 30°C, cofactor: NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 6.1 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NAD+, recombinant wild-type enzyme | Azospirillum brasilense | |
1.1.1.376 | 9.7 | - |
D-talose | pH 9.0, 30°C, cofactor: NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 12 | - |
D-xylose | pH 9.0, 30°C, cofactor: NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 16.5 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NADP+, recombinant wild-type enzyme | Azospirillum brasilense | |
1.1.1.376 | 19 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 19.8 | - |
D-galactose | pH 9.0, 30°C, cofactor: NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 26 | - |
D-galactose | pH 9.0, 30°C, cofactor: NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 33.3 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.376 | 9 | - |
assay at | Azospirillum brasilense |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.376 | NAD+ | the enzyme prefers NADP+ over NAD+ | Azospirillum brasilense | |
1.1.1.376 | NADP+ | the enzyme prefers NADP+ over NAD+ | Azospirillum brasilense |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.1.1.376 | Azospirillum brasilense | the gene is induced by L-arabinose but not by D-galactose | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.376 | malfunction | a disruptant of the L-arabinose 1-dehydrogenase gene does not grow on L-arabinose but grows on D-galactose at the same growth rate as the wild-type strain | Azospirillum brasilense |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.376 | 0.01 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NADP+, mutant enzyme N172A | Azospirillum brasilense | |
1.1.1.376 | 0.019 | - |
D-xylose | pH 9.0, 30°C, cofactor: NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 0.03 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NAD+, mutant enzyme N172A | Azospirillum brasilense | |
1.1.1.376 | 0.17 | - |
D-xylose | pH 9.0, 30°C, cofactor: NADP*, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 0.26 | - |
D-talose | pH 9.0, 30°C, cofactor: NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 1.6 | - |
D-talose | pH 9.0, 30°C, cofactor: NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 7.78 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NAD+, recombinant wild-type enzyme | Azospirillum brasilense | |
1.1.1.376 | 13.3 | - |
D-galactose | pH 9.0, 30°C, cofactor: NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 13.4 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NAD+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 63.5 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NADP+, recombinant wild-type enzyme | Azospirillum brasilense | |
1.1.1.376 | 131 | - |
L-arabinose | pH 9.0, 30°C, cofactor: NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense | |
1.1.1.376 | 240 | - |
D-galactose | pH 9.0, 30°C, cofactor: NADP+, enzyme purified from Azospirillum brasilense | Azospirillum brasilense |