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Literature summary extracted from

  • Supangat, S.; Seo, K.H.; Choi, Y.K.; Park, Y.S.; Son, D.; Han, C.D.; Lee, K.H.
    Structure of Chlorobium tepidum sepiapterin reductase complex reveals the novel substrate binding mode for stereospecific production of L-threo-tetrahydrobiopterin (2006), J. Biol. Chem., 281, 2249-2256.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.153 purified native and selenomethionine-labeled enzyme alone and in complex with NADP+ and sepiapterin, hanging drop vapour diffusion method, 18°C, 0.004 ml protein solution, with or without 10 mM NADP+ and sepiapterin, is mixed with 0.001 ml of a mixture of 0.004 ml or reservoir solution containing 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, and 34% PEG 400, and 0.001 ml additive solution 1 M guanidinium hydrochloride, X-ray diffraction structure determination and analysis at 2.1 A and 1.7 A resolution, respectively Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.153 6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+ Chlorobaculum tepidum last step of the tetrahydrobiopterin biosynthesis L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.153 Chlorobaculum tepidum
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-
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Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.153 L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+ active site structure, substrate binding mode for stereospecific production of L-threo-tetrahydrobiopterin, stereospecific hydride transfer, mechanism, structure-function relationship, overview Chlorobaculum tepidum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.153 6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+ last step of the tetrahydrobiopterin biosynthesis Chlorobaculum tepidum L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+
-
 ?
1.1.1.153 6-pyruvoyl tetrahydropterin + 2 NADPH + 2 H+ substrate binding mode for stereospecific production of L-threo-tetrahydrobiopterin, structure-function relationship Chlorobaculum tepidum L-threo-(6R,1'S,2'S)-5,6,7,8-tetrahydrobiopterin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.153 additional information structure-function relationship, amino acid sequence comparison of several species Chlorobaculum tepidum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.153 SR
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 Chlorobaculum tepidum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.153 NADPH determination and analysis of the binding structure of NADPH and NADP+ to the enzyme, overview Chlorobaculum tepidum