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Literature summary extracted from
- Protective mechanisms against homocysteine toxicity: the role of bleomycin hydrolase (2006), J. Biol. Chem., 281, 22485-22492.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.22.40 | expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
| 3.4.22.40 | expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.22.40 | C73A | inactive mutant | Homo sapiens |
| 3.4.22.40 | H369A | inactive mutant | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.40 | Arg-Ala | 86% inhibition of HLTase activity at 2 mM | Saccharomyces cerevisiae |  |
| 3.4.22.40 | Cd2+ | 5% inhibition of HLTase activity 2 mM | Saccharomyces cerevisiae | |
| 3.4.22.40 | Cu2+ | 9% inhibition of HLTase activity 2 mM | Saccharomyces cerevisiae | |
| 3.4.22.40 | E64 | 15% inhibition of HLTase activity at 0.012 mM, 97% at 0.125 mM | Homo sapiens | |
| 3.4.22.40 | E64 | 99% inhibition of HLTase activity at 0.012 mM | Saccharomyces cerevisiae | |
| 3.4.22.40 | H2O2 | 41% inhibition of HLTase activity 2 mM | Saccharomyces cerevisiae | |
| 3.4.22.40 | iodoacetamide | complete inhibition of HLTase activity at 2 mM | Homo sapiens | |
| 3.4.22.40 | iodoacetamide | 94% inhibition of HLTase activity 2 mM | Saccharomyces cerevisiae | |
| 3.4.22.40 | Leu-Ala | 50% inhibition of HLTase activity at 2 mM | Saccharomyces cerevisiae | |
| 3.4.22.40 | Lys-Ala | 77% inhibition of HLTase activity at 2 mM | Saccharomyces cerevisiae | |
| 3.4.22.40 | Lys-Leu | 26% inhibition of HLTase activity at 2 mM | Saccharomyces cerevisiae | |
| 3.4.22.40 | Zn2+ | 70% inhibition of HLTase activity at 2 mM | Saccharomyces cerevisiae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.22.40 | intracellular | - |
Homo sapiens | 5622 | - |
| 3.4.22.40 | intracellular | - |
Saccharomyces cerevisiae | 5622 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.40 | additional information | Homo sapiens | the enzyme exhibits protection against homocysteine toxicity, role and mechanisms of bleomycin hydrolase, overview | ? | - |
? | |
| 3.4.22.40 | additional information | Saccharomyces cerevisiae | the enzyme exhibits protection against homocysteine toxicity, role and mechanisms of bleomycin hydrolase, overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.40 | Homo sapiens | - |
- |
- |
| 3.4.22.40 | Saccharomyces cerevisiae | - |
several strains | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.40 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity | Homo sapiens |
| 3.4.22.40 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.40 | additional information | substrate specificity, overview | Homo sapiens | ? | - |
? | |
| 3.4.22.40 | additional information | substrate specificity, overview | Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.22.40 | additional information | the enzyme exhibits protection against homocysteine toxicity, role and mechanisms of bleomycin hydrolase, overview | Homo sapiens | ? | - |
? | |
| 3.4.22.40 | additional information | the enzyme exhibits protection against homocysteine toxicity, role and mechanisms of bleomycin hydrolase, overview | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.22.40 | BLH | - |
Homo sapiens |
| 3.4.22.40 | Hcy-thiolactonase | the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview | Homo sapiens |
| 3.4.22.40 | Hcy-thiolactonase | the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview | Saccharomyces cerevisiae |
| 3.4.22.40 | homocysteine thiolactonase | the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview | Homo sapiens |
| 3.4.22.40 | homocysteine thiolactonase | the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview | Saccharomyces cerevisiae |
| 3.4.22.40 | HTLase | the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview | Homo sapiens |
| 3.4.22.40 | HTLase | the bleomycin hydrolase is identical with the homocysteine thiolactonase, overview | Saccharomyces cerevisiae |
| 3.4.22.40 | yBLH | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.40 | 25 | - |
assay at | Homo sapiens |
| 3.4.22.40 | 25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.40 | 7.4 | - |
assay at | Homo sapiens |
| 3.4.22.40 | 7.4 | - |
assay at | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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