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Literature summary extracted from

  • Simon-Nobbe, B.; Denk, U.; Schneider, P.B.; Radauer, C.; Teige, M.; Crameri, R.; Hawranek, T.; Lang, R.; Richter, K.; Schmid-Grendelmeier, P.; Nobbe, S.; Hartl, A.; Breitenbach, M.
    NADP-dependent mannitol dehydrogenase, a major allergen of Cladosporium herbarum (2006), J. Biol. Chem., 281, 16354-16360.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.138 expressed in E. coli Cladosporium herbarum
1.1.1.138 gene MtDH, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Cladosporium herbarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.138 0.053
-
 NADP+ pH 7.5, 22°C Cladosporium herbarum
1.1.1.138 0.053
-
 NADPH plusminus 0.01 mM, pH 7.5, room temperature Cladosporium herbarum
1.1.1.138 0.067
-
 NADPH pH 7.5, 22°C Cladosporium herbarum
1.1.1.138 0.067
-
 NADP+ plusminus 0.007 mM, pH 7.5, room temperature Cladosporium herbarum
1.1.1.138 33
-
 D-mannitol plusminus 5 mM, pH 7.5, room temperature Cladosporium herbarum
1.1.1.138 117
-
 D-fructose plusminus 20 mM, pH 7.5, room temperature Cladosporium herbarum
1.1.1.138 230
-
 D-mannitol pH 7.5, 22°C Cladosporium herbarum
1.1.1.138 1170
-
 D-fructose pH 7.5, 22°C Cladosporium herbarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.138 28320
-
-
 Cladosporium herbarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.138 D-mannitol + NADP+ Cladosporium herbarum
-
 D-fructose + NADPH + H+
-
 r
1.1.1.138 additional information Cladosporium herbarum the enzyme is the major allergen in the organism causing allergic reaction e.g. in humans ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.138 Cladosporium herbarum P0C0Y5
-
-
1.1.1.138 Cladosporium herbarum P0C0Y5 i.e. Davidiella tassiana, strain 280202
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.138 native enzyme 13.4fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, and anion exchange chromatography Cladosporium herbarum
1.1.1.138 purified by standard chromatographic methods Cladosporium herbarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.138 additional information
-
 allergenic potential on human skin, overview Cladosporium herbarum
1.1.1.138 277
-
 purified native enzyme Cladosporium herbarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.138 D-fructose + NADPH + H+
-
 Cladosporium herbarum D-mannitol + NADP+
-
 r
1.1.1.138 D-mannitol + NADP+
-
 Cladosporium herbarum D-fructose + NADPH + H+
-
 r
1.1.1.138 additional information the enzyme is the major allergen in the organism causing allergic reaction e.g. in humans Cladosporium herbarum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.138 Cla h 8
-
 Cladosporium herbarum
1.1.1.138 Cla h 8 allergen
-
 Cladosporium herbarum
1.1.1.138 MtDH
-
 Cladosporium herbarum
1.1.1.138 NADP-dependent mannitol dehydrogenase
-
 Cladosporium herbarum
1.1.1.138 NADP-dependent MtDH
-
 Cladosporium herbarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.138 22
-
 assay at room temperature, forward reaction Cladosporium herbarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.138 7.5
-
 assay at, forward reaction Cladosporium herbarum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.138 NADP+
-
 Cladosporium herbarum
1.1.1.138 NADPH
-
 Cladosporium herbarum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.138 Cladosporium herbarum
-
-
 5.8