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Literature summary extracted from

  • Naqvi, N.; Liu, K.; Graham, R.M.; Husain, A.
    Molecular basis of exopeptidase activity in the C-terminal domain of human angiotensin I-converting enzyme: insights into the origins of its exopeptidase activity (2005), J. Biol. Chem., 280, 6669-6675.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.15.1 K1087A no activity with angiotensin I, kcat/KM for [Phe9]angiotensin I is 654fold lower than wild-type value, kcat/KM for [Arg10]angiotensin I is 193fold lower than wild-type value, kcat/KM for [Phe9,Arg10]angiotensin I is 462fold lower than wild-type value Homo sapiens
3.4.15.1 R1098Q kcat/KM for angiotensin I is 7fold lower than wild-type value, kcat/KM for [Phe9]angiotensin I is 2.2fold higher than wild-type value, kcat/KM for [Arg10]angiotensin I is 23fold lower than wild-type value, kcat/KM for [Phe9,Arg10]angiotensin I is 1.4fold lower than wild-type value Homo sapiens
3.4.15.1 Y1096F kcat/KM for angiotensin I is 12fold lower than wild-type value, kcat/KM for [Phe9]angiotensin I is 8.9fold lower than wild-type value, kcat/KM for [Arg10]angiotensin I is 8.5fold lower than wild-type value, kcat/KM for [Phe9,Arg10]angiotensin I is 7fold lower than wild-type value Homo sapiens
3.4.15.1 Y1096F/K1087A no activity with angiotensin I, [Phe9]angiotensin I, [Arg10]angiotensin I, and [Phe9,Arg10]angiotensin I Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.15.1 0.011
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
3.4.15.1 0.014
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme Y1096F Homo sapiens
3.4.15.1 0.02
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens
3.4.15.1 0.025
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme K1087A Homo sapiens
3.4.15.1 0.032
-
 [Phe9]angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
3.4.15.1 0.042
-
 [Phe9]angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens
3.4.15.1 0.044
-
 [Arg10]angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
3.4.15.1 0.046
-
 [Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme Y1096F Homo sapiens
3.4.15.1 0.052
-
 angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
3.4.15.1 0.056
-
 [Phe9]angiotensin I 37°C, pH 7.5, mutant enzyme Y1096F Homo sapiens
3.4.15.1 0.1
-
 angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens
3.4.15.1 0.22
-
 [Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.15.1 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.15.1 angiotensin I + H2O
-
 Homo sapiens angiotensin II + His-Leu
-
 ?
3.4.15.1 bradykinin + H2O
-
 Homo sapiens ?
-
 ?
3.4.15.1 [Arg10]angiotensin I + H2O
-
 Homo sapiens ?
-
 ?
3.4.15.1 [Phe9,Arg10]angiotensin I + H2O
-
 Homo sapiens ?
-
 ?
3.4.15.1 [Phe9]angiotensin I + H2O
-
 Homo sapiens ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.15.1 6.5
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme K1087A Homo sapiens
3.4.15.1 52
-
 angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
3.4.15.1 57
-
 [Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme Y1096F Homo sapiens
3.4.15.1 60
-
 angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens
3.4.15.1 100
-
 [Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens
3.4.15.1 110
-
 [Phe9]angiotensin I 37°C, pH 7.5, mutant enzyme Y1096F Homo sapiens
3.4.15.1 220
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme Y1096F Homo sapiens
3.4.15.1 460
-
 [Arg10]angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
3.4.15.1 550
-
 [Phe9]angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
3.4.15.1 1400
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
3.4.15.1 1500
-
 [Phe9]angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens
3.4.15.1 1700
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens