BRENDA - Enzyme Database

Location of the coenzyme binding site in the porcine mitochondrial NADP-dependent isocitrate dehydrogenase

Huang, Y.C.; Colman, R.F.; J. Biol. Chem. 280, 30349-30353 (2005)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
1.1.1.42
in complex with Mn2+ and isocitrate
Sus scrofa
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
N328D
36% decrease in vmax-value compared to wild-type
Sus scrofa
1.1.1.42
N328S
slight decrease in vmax-value compared to wild-type
Sus scrofa
1.1.1.42
R83K
slight decrease in vmax-value compared to wild-type
Sus scrofa
1.1.1.42
R83Q
slight decrease in vmax-value compared to wild-type
Sus scrofa
1.1.1.42
T311A
slight decrease in vmax-value compared to wild-type
Sus scrofa
1.1.1.42
T311N
vmax-value is less than 1% of the value of wild-type
Sus scrofa
1.1.1.42
T311S
large increase in vmax-value compared to wild-type
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0043
-
NADP+
mutant T311N, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0056
-
NADP+
wild-type, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0064
-
DL-isocitrate
mutant T311S, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0065
-
DL-isocitrate
mutant R83K, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0073
-
DL-isocitrate
mutant T311N, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0084
-
DL-isocitrate
wild-type, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0097
-
NADP+
mutant T311S, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0114
-
DL-isocitrate
mutant N328D, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.014
-
DL-isocitrate
mutant R83Q, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0154
-
DL-isocitrate
mutant N328S, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0155
-
DL-isocitrate
mutant T311A, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0203
-
NADP+
mutant R83K, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0246
-
NADP+
mutant N328D, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0749
-
NADP+
mutant T311A, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0811
-
NADP+
mutant R83Q, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.119
-
NADP+
mutant N328S, pH 7.4, 25°C
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
mitochondrion
-
Sus scrofa
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mn2+
wild-type, Km-value 0.00033 mM
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Sus scrofa
P33198
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
DL-isocitrate + NADP+
-
669373
Sus scrofa
2-oxoglutarate + CO2 + NADPH
-
-
-
?
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.1.1.42
in complex with Mn2+ and isocitrate
Sus scrofa
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
N328D
36% decrease in vmax-value compared to wild-type
Sus scrofa
1.1.1.42
N328S
slight decrease in vmax-value compared to wild-type
Sus scrofa
1.1.1.42
R83K
slight decrease in vmax-value compared to wild-type
Sus scrofa
1.1.1.42
R83Q
slight decrease in vmax-value compared to wild-type
Sus scrofa
1.1.1.42
T311A
slight decrease in vmax-value compared to wild-type
Sus scrofa
1.1.1.42
T311N
vmax-value is less than 1% of the value of wild-type
Sus scrofa
1.1.1.42
T311S
large increase in vmax-value compared to wild-type
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0043
-
NADP+
mutant T311N, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0056
-
NADP+
wild-type, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0064
-
DL-isocitrate
mutant T311S, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0065
-
DL-isocitrate
mutant R83K, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0073
-
DL-isocitrate
mutant T311N, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0084
-
DL-isocitrate
wild-type, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0097
-
NADP+
mutant T311S, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0114
-
DL-isocitrate
mutant N328D, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.014
-
DL-isocitrate
mutant R83Q, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0154
-
DL-isocitrate
mutant N328S, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0155
-
DL-isocitrate
mutant T311A, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0203
-
NADP+
mutant R83K, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0246
-
NADP+
mutant N328D, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0749
-
NADP+
mutant T311A, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.0811
-
NADP+
mutant R83Q, pH 7.4, 25°C
Sus scrofa
1.1.1.42
0.119
-
NADP+
mutant N328S, pH 7.4, 25°C
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
mitochondrion
-
Sus scrofa
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mn2+
wild-type, Km-value 0.00033 mM
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
DL-isocitrate + NADP+
-
669373
Sus scrofa
2-oxoglutarate + CO2 + NADPH
-
-
-
?