EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.6.4.13 | additional information | NS3 requires the hydrophilic domain of NS2B for activation | Dengue virus type 2 | |
3.6.4.13 | nonstructural protein 5 | interaction with NS5, the viral RNA-dependent RNA polymerase, stimulates NS3 NTPAse and RTPase activities as well as thr RNA helicase activity | Dengue virus type 2 | |
3.6.4.13 | RNA | RNA-stimulated enzyme | Dengue virus type 2 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.4.13 | expression of wild-type and N-terminally truncated enzyme in Escherichia coli | Dengue virus type 2 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.4.13 | H51A | site-directed mutagenesis, the mutant has an inactivated protease domain showing enhanced RNA helicase compared to wild-type full-length enzyme | Dengue virus type 2 |
3.6.4.13 | R184Q/K185N/R186G/K187N | construction of the N-terminally truncated mutant NS3DELTA180 containing a mutated RNA substrate biding motif, the mutant shows reduced RTPase activity | Dengue virus type 2 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.4.13 | additional information | - |
additional information | kinetic study | Dengue virus type 2 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.4.13 | Mg2+ | - |
Dengue virus type 2 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.4.13 | ATP + H2O | Dengue virus type 2 | RNA helicase activity | ADP + phosphate | - |
? | |
3.6.4.13 | additional information | Dengue virus type 2 | nonstructural proteins NS3 and NS5 form complexes in infected mammalian cells | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.4.13 | Dengue virus type 2 | - |
i.e. DEN2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.4.13 | recombinant wild-type and N-terminally truncated enzyme from Escherichia coli | Dengue virus type 2 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.4.13 | additional information | - |
specificities and activities of wild-type and mutant enzymes | Dengue virus type 2 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.4.13 | ATP + H2O | NTPase activity | Dengue virus type 2 | ADP + phosphate | - |
? | |
3.6.4.13 | ATP + H2O | RNA helicase activity | Dengue virus type 2 | ADP + phosphate | - |
? | |
3.6.4.13 | additional information | nonstructural proteins NS3 and NS5 form complexes in infected mammalian cells | Dengue virus type 2 | ? | - |
? | |
3.6.4.13 | additional information | substrate specificity, bifunctional enzyme, NS3 is an RNA-stimulated nucleoside triphosphatase NTPase/RNA helicase and a 5'-RNA triphosphatase RTPase, overview, the full-length NS3 with or without NS2B cofactor domain exhibits a catalytically more efficient RNA helicase activity than the N-terminally-truncated NS3 helicase domain, suggesting that the protease domain enhances RNA helicase activity | Dengue virus type 2 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.4.13 | More | DEN2 nonstructural protein 3, NS3, has a serine protease domain and requires the hydrophilic domain of NS2B for activation | Dengue virus type 2 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.4.13 | nonstructural protein 3 | - |
Dengue virus type 2 |
3.6.4.13 | NS3 | - |
Dengue virus type 2 |
3.6.4.13 | nucleoside triphosphatase/RNA helicase and 5'-RNA triphosphatase | - |
Dengue virus type 2 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.4.13 | 37 | - |
assay at | Dengue virus type 2 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.4.13 | 7.5 | - |
assay at | Dengue virus type 2 |