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Literature summary extracted from

  • Song, E.S.; Daily, A.; Fried, M.G.; Juliano, M.A.; Juliano, L.; Hersh, L.B.
    Mutation of active site residues of insulin-degrading enzyme alters allosteric interactions (2005), J. Biol. Chem., 280, 17701-17706.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.56 E111A change in the substrate response from sigmoidal to hyperbolic. Activating effect of ATP or triphosphate with substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) on wild-type is dampened. Mutant recognizes additional cleavage sites in substrate beta-endorphin Rattus norvegicus
3.4.24.56 E111F change in the substrate response from sigmoidal to hyperbolic. Activating effect of ATP or triphosphate with substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) on wild-type is dampened. Mutant recognizes additional cleavage sites in substrate beta-endorphin Rattus norvegicus
3.4.24.56 E111L change in the substrate response from sigmoidal to hyperbolic. Activating effect of ATP or triphosphate with substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) on wild-type is dampened. Mutant recognizes additional cleavage sites in substrate beta-endorphin Rattus norvegicus
3.4.24.56 E111V change in the substrate response from sigmoidal to hyperbolic. Activating effect of ATP or triphosphate with substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) on wild-type is dampened. Mutant recognizes additional cleavage sites in substrate beta-endorphin Rattus norvegicus
3.4.24.56 H112D change in the substrate response from sigmoidal to hyperbolic. Activating effect of ATP or triphosphate with substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) on wild-type is changed to inhibition. Affinity to Zn2+ is lower than in wild-type Rattus norvegicus
3.4.24.56 H112Q change in the substrate response from sigmoidal to hyperbolic. Activating effect of ATP or triphosphate with substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) on wild-type is changed to inhibition. Affinity to Zn2+ is lower than in wild-type Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.56 EDTA 0.1 mM, wild-type, 91% residual activity, mutants H112D, H112Q, less than 2.5% residual activity Rattus norvegicus
3.4.24.56 o-phenanthroline 0.1 mM, wild-type, 73% residual activity, mutants H112D, H112Q, less than 2.5% residual activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.56 0.014
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111L, pH 7.3 Rattus norvegicus
3.4.24.56 0.0156
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111V, pH 7.3 Rattus norvegicus
3.4.24.56 0.0274
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111F, pH 7.3 Rattus norvegicus
3.4.24.56 0.0287
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) wild-type, K0.5-value, pH 7.3 Rattus norvegicus
3.4.24.56 0.083
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant H112D, pH 7.3 Rattus norvegicus
3.4.24.56 0.158
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant H112Q, pH 7.3 Rattus norvegicus
3.4.24.56 0.204
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111A, pH 7.3 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.56 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.56 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.56 amyloid beta-protein + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.56 beta-endorphin + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.24.56 insulin + H2O
-
 Rattus norvegicus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.56 2 3.7 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant H112D, pH 7.3 Rattus norvegicus
3.4.24.56 2.4
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111V, pH 7.3 Rattus norvegicus
3.4.24.56 6.5
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111L, pH 7.3 Rattus norvegicus
3.4.24.56 11.4
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111F, pH 7.3 Rattus norvegicus
3.4.24.56 17.5
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant E111A, pH 7.3 Rattus norvegicus
3.4.24.56 1953
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) mutant H112Q, pH 7.3 Rattus norvegicus
3.4.24.56 162600
-
 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) wild-type, pH 7.3 Rattus norvegicus