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Literature summary extracted from

  • Michael, I.P.; Sotiropoulou, G.; Pampalakis, G.; Magklara, A.; Ghosh, M.; Wasney, G.; Diamandis, E.P.
    Biochemical and enzymatic characterization of human kallikrein 5 (hK5), a novel serine protease potentially involved in cancer progression (2005), J. Biol. Chem., 280, 14628-14635.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B39 expression in Pichia pastoris Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B39 alpha2-antiplasmin 50% inhibition at 0.8 microM in 0.6 min Homo sapiens
3.4.21.B39 antithrombin III 50% inhibition at 0.8 microM in 6 min Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B39 0.02
-
 Boc-Val-Pro-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.15
-
 Boc-Leu-GLy-Arg-7-amido-4-methylcoumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.19
-
 Boc-Phe-Ser-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.31
-
 Z-Gly-Gly-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.48
-
 Boc-Leu-Arg-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.57
-
 Boc-Gln-Gly-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.61
-
 Boc-Gln-Ala-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.64
-
 Boc-Val-Leu-Lys-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 1.01
-
 Boc-Leu-Lys-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 1.41
-
 Boc-Gln-Arg-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 1.69
-
 Tos-Gly-Pro-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 1.77
-
 H-Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B39 26000
-
 calculated from sequence Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B39 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.B39 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B39 two chromatography steps, 95% purity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B39 Boc-Gln-Ala-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Gln-Ala-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.B39 Boc-Gln-Arg-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Gln-Arg-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.B39 Boc-Gln-Gly-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Gln-Gly-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.B39 Boc-Leu-Arg-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Leu-Arg-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.B39 Boc-Leu-GLy-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Boc-Leu-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B39 Boc-Leu-Lys-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Leu-Lys-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.B39 Boc-Phe-Ser-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Phe-Ser-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.B39 Boc-Val-Leu-Lys-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Val-Leu-Lys + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.B39 Boc-Val-Pro-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Boc-Val-Pro-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.B39 casein + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.B39 fibrinogen Aalpha chain + H2O cleavage after Arg348 and Arg407 Homo sapiens ?
-
 ?
3.4.21.B39 fibrinogen Bbeta chain + H2O cleavage after Lys53 and Arg14 Homo sapiens ?
-
 ?
3.4.21.B39 Gelatin + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.B39 H-Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens H-Pro-Phe-Ser-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.B39 kininogen + H2O cleavage after Arg222, Arg252 and Arg374 Homo sapiens ?
-
 ?
3.4.21.B39 plasminogen + H2O cleavage after Lys77 andA rg529 Homo sapiens ?
-
 ?
3.4.21.B39 Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Pro-Phe-Ser-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.B39 Tos-Gly-Pro-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Tos-Gly-Pro-Arg + 7-amino-4-methyl-coumarin
-
 ?
3.4.21.B39 Vitronectin + H2O cleavage after Arg8 Homo sapiens ?
-
 ?
3.4.21.B39 Z-Gly-Gly-Arg-7-amido-4-methyl-coumarin + H2O
-
 Homo sapiens Z-Gly-Gly-Arg + 7-amino-4-methyl-coumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B39 hK5
-
 Homo sapiens
3.4.21.B39 human kallikrein 5
-
 Homo sapiens
3.4.21.B39 KLK5
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B39 0.04
-
 Z-Gly-Gly-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.07
-
 Boc-Leu-GLy-Arg-7-amido-4-methylcoumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.08
-
 Boc-Val-Leu-Lys-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.18
-
 Boc-Gln-Gly-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.31
-
 Boc-Leu-Arg-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.35
-
 Tos-Gly-Pro-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.41
-
 Boc-Gln-Arg-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.47
-
 H-Pro-Phe-Ser-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 0.82
-
 Boc-Leu-Lys-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 1.78
-
 Boc-Gln-Ala-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 2.83
-
 Boc-Phe-Ser-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens
3.4.21.B39 3.28
-
 Boc-Val-Pro-Arg-7-amido-4-methyl-coumarin 100 mM phosphate, 0.01% Tween 20, pH 8.0, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B39 8
-
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.B39 0.00107
-
 alpha2-antiplasmin
-
 Homo sapiens
3.4.21.B39 0.0091
-
 antithrombin III
-
 Homo sapiens