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Literature summary extracted from

  • Narasaki, R.; Kuribayashi, H.; Shimizu, K.; Imamura, D.; Sato, T.; Hasumi, K.
    Bacillolysin MA, a novel bacterial metalloproteinase that produces angiostatin-like fragments from plasminogen and activates protease zymogens in the coagulation and fibrinolysis systems (2005), J. Biol. Chem., 280, 14278-14287.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.28 CoCl2 0.3 mM, 1.8fold increase in activity Priestia megaterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.28 EDTA 1 mM, complete inhibition Priestia megaterium
3.4.24.28 additional information not inhibitory: 1 mM iodoacetic acid, 1 mM phenylmethylsulfonyl fluoride, 0.5 mM leupeptin, 0.5 mM pepstatin, 0.005 mM alpah2-plasmin inhibitor Priestia megaterium
3.4.24.28 Zn2+ 1 mM, 80% loss of activity Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.28 0.003
-
 plasminogen pH 7.4, 25°C Priestia megaterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.28 35300
-
 x * 35300, SDS-PAGE Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.28 Priestia megaterium
-
 A9542
-
3.4.24.28 Priestia megaterium A9542
-
 A9542
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.28 3-(2-furylacryloyl)-glycyl-L-leucine amide + H2O
-
 Priestia megaterium ?
-
 ?
3.4.24.28 3-(2-furylacryloyl)-glycyl-L-leucine amide + H2O
-
 Priestia megaterium A9542 ?
-
 ?
3.4.24.28 casein + H2O
-
 Priestia megaterium ?
-
 ?
3.4.24.28 casein + H2O
-
 Priestia megaterium A9542 ?
-
 ?
3.4.24.28 coagulation factor X + H2O
-
 Priestia megaterium ?
-
 ?
3.4.24.28 coagulation factor X + H2O
-
 Priestia megaterium A9542 ?
-
 ?
3.4.24.28 Gelatin + H2O
-
 Priestia megaterium ?
-
 ?
3.4.24.28 Gelatin + H2O
-
 Priestia megaterium A9542 ?
-
 ?
3.4.24.28 plasminogen + H2O cleavage mainly at S441-V442 Priestia megaterium angiostatin + mini-plasminogen
-
 ?
3.4.24.28 protein C + H2O
-
 Priestia megaterium ?
-
 ?
3.4.24.28 protein C + H2O
-
 Priestia megaterium A9542 ?
-
 ?
3.4.24.28 prothrombin + H2O
-
 Priestia megaterium thrombin + thrombin propeptide
-
 ?
3.4.24.28 prourokinase + H2O
-
 Priestia megaterium urokinase + urokinase propeptide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.28 ? x * 35300, SDS-PAGE Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
3.4.24.28 BL-MA
-
 Priestia megaterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.28 60
-
-
 Priestia megaterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.28 25 60 stable Priestia megaterium
3.4.24.28 70
-
 20 min, 65% loss of activity Priestia megaterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.28 0.7
-
 plasminogen pH 7.4, 25°C Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.28 7.4
-
-
 Priestia megaterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.28 4
-
 60 min, complete loss of activity Priestia megaterium
3.4.24.28 5 9 37°C, 60 min, stable Priestia megaterium
3.4.24.28 11.7
-
 60 min, complete loss of activity Priestia megaterium