BRENDA - Enzyme Database

Oligopeptidase B from Trypanosoma evansi. A parasite peptidase that inactivates atrial natriuretic factor in the bloodstream of infected hosts

Morty, R.E.; Pelle, R.; Vadasz, I.; Uzcanga, G.L.; Seeger, W.; Bubis, J.; J. Biol. Chem. 280, 10925-10937 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.4.21.83
-
Trypanosoma evansi
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.4.21.83
S563A
catalytically inactive OpdB variant, no activity with benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin and tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.21.83
3,4-dichloroisocoumarin
irreversible
Trypanosoma evansi
3.4.21.83
4-(2-aminoethyl)benzenesulfonyl fluoride
irreversible
Trypanosoma evansi
3.4.21.83
agmatine
reversible
Trypanosoma evansi
3.4.21.83
antipain
reversible
Trypanosoma evansi
3.4.21.83
benzamidine
reversible
Trypanosoma evansi
3.4.21.83
leupeptin
reversible
Trypanosoma evansi
3.4.21.83
phenylmethanesulfonyl fluoride
irreversible
Trypanosoma evansi
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.21.83
0.0002
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme; pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0002
-
Val-Lys-Arg-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0003
-
Val-Lys-Arg-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0006
-
Arg-Arg-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0008
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.001
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.001
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0011
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0011
-
Arg-Arg-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0011
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0012
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0012
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0013
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0013
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0016
-
Gly-Gly-Lys-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0017
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0019
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0019
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.002
-
Arg-Arg-2-naphthylamide
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0021
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0021
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0021
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0022
-
Gly-Gly-Lys-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0023
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0024
-
Arg-Arg-2-naphthylamide
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0027
-
Phe-Arg-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0029
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.003
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.003
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.003
-
Phe-Arg-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.003
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.003
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0033
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0038
-
Phe-Arg-2-naphthylamide
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.004
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0044
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0044
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0047
-
Gly-Arg-2-naphthylamide
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0048
-
Gly-Arg-2-naphthylamide
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0049
-
Phe-Arg-2-naphthylamide
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.005
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0102
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0122
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0135
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0143
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.04
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.048
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.4.21.83
Atrial natriuretic factor + H2O
Trypanosoma evansi
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.4.21.83
Trypanosoma evansi
Q6QDP1
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.4.21.83
-
Trypanosoma evansi
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.21.83
additional information
Trypanosoma evansi releases oligopeptidase B into the plasma of infected hosts
Trypanosoma evansi
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.21.83
0.017
-
-
Trypanosoma evansi
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.21.83
3-(2-furyl)acryloyl-Tyr-Lys-Arg + H2O
-
669308
Trypanosoma evansi
3-(2-furyl)acryloyl-Tyr-Lys + Arg
-
-
-
?
3.4.21.83
Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Arg-Arg-2-naphthylamide + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Arg-Arg-Arg-OH + H2O
-
669308
Trypanosoma evansi
Arg-Arg + Arg
-
-
-
?
3.4.21.83
Atrial natriuretic factor + H2O
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Atrial natriuretic factor + H2O
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Gly-Arg-2-naphthylamide + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Gly-Gly-Lys-Arg + H2O
-
669308
Trypanosoma evansi
Gly-Gly-Lys-Arg + Arg
-
-
-
?
3.4.21.83
Phe-Arg-2-naphthylamide + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Phe-Arg-Arg + H2O
-
669308
Trypanosoma evansi
Phe-Arg + Arg
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Val-Lys-Arg-Arg + H2O
-
669308
Trypanosoma evansi
Val-Lys-Arg + Arg
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.21.83
1
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
2
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
8
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
9
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
11
-
Gly-Arg-2-naphthylamide
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
12
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
12
-
Gly-Arg-2-naphthylamide
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
12
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
12
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
16
-
Phe-Arg-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
17
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
17
-
Phe-Arg-2-naphthylamide
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
18
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
18
-
Phe-Arg-2-naphthylamide
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
18
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
21
-
Arg-Arg-2-naphthylamide
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
21
-
Phe-Arg-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
22
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
23
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
26
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
29
-
Arg-Arg-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
30
-
Arg-Arg-2-naphthylamide
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
33
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C,recombinant enzyme
Trypanosoma evansi
3.4.21.83
34
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
38
-
Arg-Arg-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
38
-
Val-Lys-Arg-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
40
-
Gly-Gly-Lys-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
44
-
Val-Lys-Arg-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
48
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
54
-
Gly-Gly-Lys-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
55
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C,recombinant enzyme
Trypanosoma evansi
3.4.21.83
57
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
58
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
59
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
59
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
64
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
64
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
74
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
76
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
77
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
81
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
94
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
101
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
102
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
108
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
122
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, ecombinant mutant enzyme S563A
Trypanosoma evansi
3.4.21.83
132
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
154
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.21.83
8.5
-
hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.21.83
7
10.5
pH 7.0: about 50% of maximal activity, pH 10.5: about 50% of maximal activity, hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.4.21.83
0.001
-
antipain
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.002
-
antipain
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.021
-
leupeptin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.029
-
leupeptin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.188
-
agmatine
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.207
-
agmatine
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.218
-
benzamidine
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.254
-
benzamidine
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.21.83
-
Trypanosoma evansi
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.4.21.83
S563A
catalytically inactive OpdB variant, no activity with benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin and tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.21.83
3,4-dichloroisocoumarin
irreversible
Trypanosoma evansi
3.4.21.83
4-(2-aminoethyl)benzenesulfonyl fluoride
irreversible
Trypanosoma evansi
3.4.21.83
agmatine
reversible
Trypanosoma evansi
3.4.21.83
antipain
reversible
Trypanosoma evansi
3.4.21.83
benzamidine
reversible
Trypanosoma evansi
3.4.21.83
leupeptin
reversible
Trypanosoma evansi
3.4.21.83
phenylmethanesulfonyl fluoride
irreversible
Trypanosoma evansi
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.4.21.83
0.001
-
antipain
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.002
-
antipain
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.021
-
leupeptin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.029
-
leupeptin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.188
-
agmatine
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.207
-
agmatine
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.218
-
benzamidine
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.254
-
benzamidine
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.21.83
0.0002
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme; pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0002
-
Val-Lys-Arg-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0003
-
Val-Lys-Arg-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0006
-
Arg-Arg-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0008
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.001
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.001
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0011
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0011
-
Arg-Arg-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0011
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0012
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0012
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0013
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0013
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0016
-
Gly-Gly-Lys-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0017
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0019
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0019
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.002
-
Arg-Arg-2-naphthylamide
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0021
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0021
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0021
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0022
-
Gly-Gly-Lys-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0023
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0024
-
Arg-Arg-2-naphthylamide
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0027
-
Phe-Arg-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0029
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.003
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.003
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.003
-
Phe-Arg-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.003
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.003
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0033
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0038
-
Phe-Arg-2-naphthylamide
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.004
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0044
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0044
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0047
-
Gly-Arg-2-naphthylamide
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0048
-
Gly-Arg-2-naphthylamide
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0049
-
Phe-Arg-2-naphthylamide
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.005
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0102
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.0122
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0135
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.0143
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
0.04
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
0.048
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.4.21.83
Atrial natriuretic factor + H2O
Trypanosoma evansi
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.21.83
-
Trypanosoma evansi
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.21.83
additional information
Trypanosoma evansi releases oligopeptidase B into the plasma of infected hosts
Trypanosoma evansi
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.21.83
0.017
-
-
Trypanosoma evansi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.21.83
3-(2-furyl)acryloyl-Tyr-Lys-Arg + H2O
-
669308
Trypanosoma evansi
3-(2-furyl)acryloyl-Tyr-Lys + Arg
-
-
-
?
3.4.21.83
Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Arg-Arg-2-naphthylamide + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Arg-Arg-Arg-OH + H2O
-
669308
Trypanosoma evansi
Arg-Arg + Arg
-
-
-
?
3.4.21.83
Atrial natriuretic factor + H2O
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor. The in vitro half-life of atrial natriuretic factor in rat plasma is reduced 300fold in plasma from Trypanosoma evansi infected rodents, which contain high levels of OpdB activity. Oligopeptidase B plays a role in peptide hormone dysregulation in trypanosomiasis, specifically in generating the depressed plasma levels of atrial natriuretic factor in mammals infected with trypanosoma evansi
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Atrial natriuretic factor + H2O
cleavage at four sites: Arg3-Arg4, Arg4-/-Ser5, Arg11-/-Ile12, and Arg27-Tyr28, thereby abrogating smooth muscle relaxant and prohypotensive properties of atrial natriuretic factor
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Gly-Arg-2-naphthylamide + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Gly-Gly-Lys-Arg + H2O
-
669308
Trypanosoma evansi
Gly-Gly-Lys-Arg + Arg
-
-
-
?
3.4.21.83
Phe-Arg-2-naphthylamide + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Phe-Arg-Arg + H2O
-
669308
Trypanosoma evansi
Phe-Arg + Arg
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
669308
Trypanosoma evansi
?
-
-
-
?
3.4.21.83
Val-Lys-Arg-Arg + H2O
-
669308
Trypanosoma evansi
Val-Lys-Arg + Arg
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.21.83
1
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
2
-
Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
8
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
9
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
11
-
Gly-Arg-2-naphthylamide
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
12
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
12
-
Gly-Arg-2-naphthylamide
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
12
-
tert-butyloxycarbonyl-Ala-Gly-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
12
-
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
16
-
Phe-Arg-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
17
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
17
-
Phe-Arg-2-naphthylamide
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
18
-
3-(2-furyl)acryloyl-Tyr-Lys-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
18
-
Phe-Arg-2-naphthylamide
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
18
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
21
-
Arg-Arg-2-naphthylamide
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
21
-
Phe-Arg-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
22
-
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
23
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
26
-
benzyloxycarbonyl-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
29
-
Arg-Arg-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
30
-
Arg-Arg-2-naphthylamide
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
33
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C,recombinant enzyme
Trypanosoma evansi
3.4.21.83
34
-
Ala-Phe-Lys-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
38
-
Arg-Arg-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
38
-
Val-Lys-Arg-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
40
-
Gly-Gly-Lys-Arg
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
44
-
Val-Lys-Arg-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
48
-
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
54
-
Gly-Gly-Lys-Arg
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
55
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C,recombinant enzyme
Trypanosoma evansi
3.4.21.83
57
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
58
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
59
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
59
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
64
-
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
64
-
tert-butyloxycarbonyl-Val-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
74
-
tert-butyloxycarbonyl-Leu-Gly-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
76
-
tert-butyloxycarbonyl-Gly-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
77
-
benzyloxycarbonyl-Phe-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
81
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
94
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
101
-
tert-butyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
102
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
108
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, native enzyme
Trypanosoma evansi
3.4.21.83
122
-
tert-butyloxycarbonyl-Ala-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, ecombinant mutant enzyme S563A
Trypanosoma evansi
3.4.21.83
132
-
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
3.4.21.83
154
-
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
pH 8.0, 37°C, recombinant enzyme
Trypanosoma evansi
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.21.83
8.5
-
hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.21.83
7
10.5
pH 7.0: about 50% of maximal activity, pH 10.5: about 50% of maximal activity, hydrolysis of benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
Trypanosoma evansi