Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Song, E.S.; Juliano, M.A.; Juliano, L.; Fried, M.G.; Wagner, S.L.; Hersh, L.B.
    ATP effects on insulin-degrading enzyme are mediated primarily through its triphosphate moiety (2004), J. Biol. Chem., 279, 54216-54220.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.56 ADP in Tris buffer, activation for substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine). Activation in decreasing order: ATP, triphosphate, ADP, AMP Rattus norvegicus
3.4.24.56 AMP in Tris buffer, activation for substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine). Activation in decreasing order: ATP, triphosphate, ADP, AMP Rattus norvegicus
3.4.24.56 ATP in Tris buffer, up to 20fold activation for substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine), noncompetitive activator. Activation in decreasing order: ATP, triphosphate, ADP, AMP. Up to 10fold activation with substrates bradykinin, dynorphin B-9. No activation with substrates insulin or amyloid beta-protein Rattus norvegicus
3.4.24.56 Triphosphate in Tris buffer, up to 20fold activation for substrate 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine), noncompetitive activator. Activation in decreasing order: ATP, triphosphate, ADP, AMP. Up to 10fold activation with substrates bradykinin, dynorphin B-9. No activation with substrates insulin or amyloid beta-protein Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.56 Rattus norvegicus
-
 recombinant enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.56 2-aminobenzoyl-GGFLRKHGQ-(N-(2,4-dinitrophenyl)ethylenediamine) + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.56 More sedimentation analysis shows that enzyme in Tris buffers mainly consists of monomers and dimers, in phosphate buffer there are monomers, dimers and tetramers. Tris buffer supplemented with 4 mM triphosphate contains enzyme as monomer and high oligomers, but without dimers Rattus norvegicus