Literature summary extracted from

Zaika, E.I.; Perlow, R.A.; Matz, E.; Broyde, S.; Gilboa, R.; Grollman, A.P.; Zharkov, D.O.
Substrate discrimination by formamidopyrimidine-DNA glycosylase: a mutational analysis (2004), J. Biol. Chem., 279, 4849-4861.

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.2.2.23	H89A	selective diminition of the rate of excision of 8-oxoguanine	Escherichia coli
3.2.2.23	H89A/R109A	about 10fold increase in KM-value	Escherichia coli
3.2.2.23	K217T	selective reduction of the ability to excise 8-oxoguanine from DNA	Escherichia coli
3.2.2.23	R108A	R108 is a major determinant of opposite-base specificity	Escherichia coli
3.2.2.23	R109A	binding of enzyme to damaged DNA is almost abolished	Escherichia coli

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.2.2.23	0.0001	-	DNA containing 8-oxo-guanine residues mispaired to guanine	wild-type	Escherichia coli
3.2.2.23	0.00019	-	DNA containing 8-oxo-guanine residues mispaired to thymine	wild-type	Escherichia coli
3.2.2.23	0.00061	-	DNA containing 5,6-dihydrouracil	mutant K217A	Escherichia coli
3.2.2.23	0.00068	-	DNA containing 5,6-dihydrouracil	wild-type	Escherichia coli
3.2.2.23	0.0007	-	DNA containing 5,6-dihydrouracil	mutant H89A	Escherichia coli
3.2.2.23	0.0007	-	DNA containing 5,6-dihydrouracil	mutant R108A	Escherichia coli
3.2.2.23	0.001	-	DNA containing 8-oxo-guanine residues	wild-type	Escherichia coli
3.2.2.23	0.0015	-	DNA containing 8-oxo-guanine residues	mutant R108A	Escherichia coli
3.2.2.23	0.0041	-	DNA containing 8-oxo-guanine residues	mutant H89A	Escherichia coli
3.2.2.23	0.0041	-	DNA containing 8-oxo-guanine residues	mutant K217T	Escherichia coli
3.2.2.23	0.0042	-	DNA containing 8-oxo-guanine residues mispaired to thymine	mutant R108A	Escherichia coli
3.2.2.23	0.00465	-	DNA containing 5,6-dihydrouracil	mutant R109A	Escherichia coli
3.2.2.23	0.0051	-	DNA containing 8-oxo-guanine residues mispaired to guanine	mutant R108A	Escherichia coli
3.2.2.23	0.00957	-	DNA containing 5,6-dihydrouracil	mutant H89A/R109A	Escherichia coli
3.2.2.23	0.022	-	DNA containing 8-oxo-guanine residues mispaired to adenine	wild-type	Escherichia coli
3.2.2.23	0.023	-	DNA containing 8-oxo-guanine residues mispaired to adenine	mutant R108A	Escherichia coli
3.2.2.23	0.227	-	DNA containing 8-oxo-guanine residues	mutant R109A	Escherichia coli
3.2.2.23	0.44	-	DNA containing 8-oxo-guanine residues	mutant H89A/R109A	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.2.23	Escherichia coli	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.2.23	DNA containing 5,6-dihydrouracil + H2O	-	Escherichia coli	DNA + 5,6-dihydrouracil	-	?
3.2.2.23	DNA containing 8-oxo-guanine residues + H2O	-	Escherichia coli	DNA + 8-oxoguanine	-	?
3.2.2.23	DNA containing 8-oxo-guanine residues mispaired to adenine + H2O	-	Escherichia coli	DNA + 8-oxoguanine	-	?
3.2.2.23	DNA containing 8-oxo-guanine residues mispaired to guanine + H2O	-	Escherichia coli	DNA + 8-oxoguanine	-	?
3.2.2.23	DNA containing 8-oxo-guanine residues mispaired to thymine + H2O	-	Escherichia coli	DNA + 8-oxoguanine	-	?

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.2.2.23	0.000075	-	DNA containing 8-oxo-guanine residues mispaired to adenine	mutant R108A	Escherichia coli
3.2.2.23	0.00011	-	DNA containing 8-oxo-guanine residues mispaired to adenine	wild-type	Escherichia coli
3.2.2.23	0.0003	-	DNA containing 8-oxo-guanine residues	mutant R108A	Escherichia coli
3.2.2.23	0.0003	-	DNA containing 8-oxo-guanine residues mispaired to thymine	mutant R108A	Escherichia coli
3.2.2.23	0.00038	-	DNA containing 8-oxo-guanine residues mispaired to guanine	mutant R108A	Escherichia coli
3.2.2.23	0.0035	-	DNA containing 8-oxo-guanine residues mispaired to guanine	wild-type	Escherichia coli
3.2.2.23	0.0035	-	DNA containing 8-oxo-guanine residues	mutant H89A/R109A	Escherichia coli
3.2.2.23	0.004	-	DNA containing 5,6-dihydrouracil	mutant H89A/R109A	Escherichia coli
3.2.2.23	0.004	-	DNA containing 8-oxo-guanine residues	mutant K217T	Escherichia coli
3.2.2.23	0.0047	-	DNA containing 8-oxo-guanine residues mispaired to thymine	wild-type	Escherichia coli
3.2.2.23	0.005	-	DNA containing 8-oxo-guanine residues	wild-type	Escherichia coli
3.2.2.23	0.0053	-	DNA containing 5,6-dihydrouracil	mutant R108A	Escherichia coli
3.2.2.23	0.0053	-	DNA containing 5,6-dihydrouracil	mutant R109A	Escherichia coli
3.2.2.23	0.0059	-	DNA containing 8-oxo-guanine residues	mutant R109A	Escherichia coli
3.2.2.23	0.0067	-	DNA containing 8-oxo-guanine residues	mutant H89A	Escherichia coli
3.2.2.23	0.0093	-	DNA containing 5,6-dihydrouracil	mutant K217A	Escherichia coli
3.2.2.23	0.011	-	DNA containing 5,6-dihydrouracil	wild-type	Escherichia coli
3.2.2.23	0.015	-	DNA containing 5,6-dihydrouracil	mutant H89A	Escherichia coli

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Release 2023.1 (January 2023)