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Literature summary extracted from

  • Huang, D.; Hinds, T.R.; Martinez, S.E.; Doneanu, C.; Beavo, J.A.
    Molecular determinants of cGMP binding to chicken cone photoreceptor phosphodiesterase (2004), J. Biol. Chem., 279, 48143-48151.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.35 expression in Escherichia coli Gallus gallus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.35 D166A mutation reduces cGMP affinity Gallus gallus
3.1.4.35 D169A mutant enzyme shows no binding of cGMP Gallus gallus
3.1.4.35 E124A mutation reduces cGMP affinity Gallus gallus
3.1.4.35 F123A mutant enzyme shows no binding of cGMP Gallus gallus
3.1.4.35 F123W mutant enzyme shows no binding of cGMP Gallus gallus
3.1.4.35 F123Y mutation reduces cGMP affinity Gallus gallus
3.1.4.35 K170A mutation reduces cGMP affinity Gallus gallus
3.1.4.35 S165A mutation reduces cGMP affinity Gallus gallus
3.1.4.35 S97A mutation reduces cGMP affinity Gallus gallus
3.1.4.35 T172A mutant enzyme shows no binding of cGMP Gallus gallus
3.1.4.35 T175A mutation reduces cGMP affinity Gallus gallus
3.1.4.35 T176A mutant enzyme shows no binding of cGMP Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.35 0.717
-
 3',5'-cAMP enzyme from cone Gallus gallus
3.1.4.35 0.82
-
 3',5'-cAMP enzyme from rod Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.35 Gallus gallus P52731
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.35
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.35 3',5'-cAMP + H2O very low activity Gallus gallus 5'-AMP
-
 ?
3.1.4.35 3',5'-cGMP + H2O
-
 Gallus gallus 5'-GMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.35 PDE6
-
 Gallus gallus