Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Voisin, S.; Rognan, D.; Gros, C.; Ouimet, T.
    A three-dimensional model of the neprilysin 2 active site based on the X-ray structure of neprilysin. Identification of residues involved in substrate hydrolysis and inhibitor binding of neprilysin 2 (2004), J. Biol. Chem., 279, 46172-46181.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B14 angiotensin
-
 Rattus norvegicus
3.4.24.B14 bradykinin
-
 Rattus norvegicus
3.4.24.B14 cholecystokinin(1-6)
-
 Rattus norvegicus
3.4.24.B14 cholecystokinin-8
-
 Rattus norvegicus
3.4.24.B14 Leu-Met
-
 Rattus norvegicus
3.4.24.B14 Leu-Met-NH2
-
 Rattus norvegicus
3.4.24.B14 Leu5-enkephalin
-
 Rattus norvegicus
3.4.24.B14 Leu5-enkephalin-NH2
-
 Rattus norvegicus
3.4.24.B14 Substance P
-
 Rattus norvegicus
3.4.24.B14 Val-Phe
-
 Rattus norvegicus
3.4.24.B14 Val-Phe-NH2
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.B14 0.04
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme L739G Rattus norvegicus
3.4.24.B14 0.044
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme L739G/S133G Rattus norvegicus
3.4.24.B14 0.045
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme R131M Rattus norvegicus
3.4.24.B14 0.062
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2. wild-type enzyme Rattus norvegicus
3.4.24.B14 0.185
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme S133G Rattus norvegicus
3.4.24.B14 1
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme N567G Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B14 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B14 angiotensin + H2O cleavage sites: DRVY-/-IHP-/-FHL Rattus norvegicus ?
-
 ?
3.4.24.B14 bradykinin + H2O cleavage sites: RPPGFSP-/-FR Rattus norvegicus ?
-
 ?
3.4.24.B14 cholecystokinin(1-6) + H2O cleavage site: DYMG-/-WM Rattus norvegicus ?
-
 ?
3.4.24.B14 cholecystokinin-8 + H2O cleavage site: DYMG-/-WMDF-NH2 Rattus norvegicus ?
-
 ?
3.4.24.B14 Leu5-enkephalin + H2O cleavage site: YGG-/-FL Rattus norvegicus ?
-
 ?
3.4.24.B14 Leu5-enkephalin-NH2 + H2O cleavage site: YGG-/-FL-NH2 Rattus norvegicus ?
-
 ?
3.4.24.B14 Substance P + H2O cleavage sites: RPKPQQ-/-FFG-/-LM-NH2 Rattus norvegicus ?
-
 ?
3.4.24.B14 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide + H2O
-
 Rattus norvegicus succinyl-Ala-Ala + Phe-4-methylcoumarin 7-amide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B14 NEP2
-
 Rattus norvegicus
3.4.24.B14 neprilysin 2
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.B14 0.52
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, wild-type enzyme Rattus norvegicus
3.4.24.B14 1.97
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme S133G Rattus norvegicus
3.4.24.B14 5.23
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme N567G Rattus norvegicus
3.4.24.B14 14.03
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, wild-type enzyme Rattus norvegicus
3.4.24.B14 17.3
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme R131M Rattus norvegicus
3.4.24.B14 19
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme L739G/S133G Rattus norvegicus
3.4.24.B14 30.7
-
 succinyl-Ala-Ala-Phe-4-methylcoumarin 7-amide 37°C, pH 7.2, mutant enzyme L739G Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.B14 0.002
-
 Leu5-enkephalin 37°C, pH 7.2, wild-type enzyme Rattus norvegicus
3.4.24.B14 0.003
-
 bradykinin 37°C, pH 7.2, wild-type enzyme Rattus norvegicus
3.4.24.B14 0.008
-
 angiotensin 37°C, pH 7.2, wild-type enzyme Rattus norvegicus
3.4.24.B14 0.01
-
 cholecystokinin-8 37°C, pH 7.2, wild-type enzyme Rattus norvegicus
3.4.24.B14 0.011
-
 Substance P 37°C, pH 7.2, wild-type enzyme Rattus norvegicus
3.4.24.B14 0.02
-
 Leu-Met 37°C, pH 7.2, wild-type enzyme Rattus norvegicus
3.4.24.B14 0.02
-
 Leu5-enkephalin-NH2 37°C, pH 7.2, wild-type enzyme Rattus norvegicus
3.4.24.B14 0.021
-
 Val-Phe 37°C, pH 7.2, wild-type enzyme Rattus norvegicus
3.4.24.B14 0.05
-
 cholecystokinin(1-6) 37°C, pH 7.2, wild-type enzyme Rattus norvegicus
3.4.24.B14 0.13
-
 Val-Phe-NH2 37°C, pH 7.2, wild-type enzyme Rattus norvegicus
3.4.24.B14 0.175
-
 Leu-Met-NH2 37°C, pH 7.2, wild-type enzyme Rattus norvegicus