BRENDA - Enzyme Database

Regulation of plant arginase by wounding, jasmonate, and the phytotoxin coronatine

Chen, H.; McCaig, B.C.; Melotto, M.; He, S.Y.; Howe, G.A.; J. Biol. Chem. 279, 45998-46007 (2004)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.1
2-mercaptopropionate
5 mM, isoform LeARG1, 38% residual activity, LeARG2, 38% residual activity; 5 mM, isoform LeARG1, 38% residual activity, LeARG2, 38% residual activity
Solanum lycopersicum
3.5.3.1
3-Mercaptopropionate
5 mM, isoform LeARG1, 24% residual activity, LeARG2, 26% residual activity; 5 mM, isoform LeARG1, 24% residual activity, LeARG2, 26% residual activity
Solanum lycopersicum
3.5.3.1
Mercaptoacetate
5 mM, isoform LeARG1, 28% residual activity, LeARG2, 26% residual activity; 5 mM, isoform LeARG1, 28% residual activity, LeARG2, 26% residual activity
Solanum lycopersicum
3.5.3.1
Nomega-hydroxy-L-arginine
0.2 mM, isoform LeARG1, 7% residual activity, LeARG2, 5% residual activity; 0.2 mM, isoform LeARG1, 7% residual activity, LeARG2, 5% residual activity
Solanum lycopersicum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.1
37000
-
x * 37000, SDS-PAGE and calculated; x * 37000, SDS-PAGE and calculated
Solanum lycopersicum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.1
Solanum lycopersicum
Q5UNS1
isoform LeARG2; isoform LeARG2
-
3.5.3.1
Solanum lycopersicum
Q5UNS2
isoform LeARG1; enzyme isoform LeARG1
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.1
leaf
induction of isoform LeARG2 upon wounding and treatment with jasmonic acid
Solanum lycopersicum
-
3.5.3.1
additional information
highest levels of enzyme activity in reproductive tissues
Solanum lycopersicum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.1
agmatine + H2O
isoform LeARG1, 0.5% of the activity with L-arginine, LeARG2, 0.6% of the activity with L-arginine
669279
Solanum lycopersicum
1,4-diaminobutane + urea
-
-
-
?
3.5.3.1
D-arginine + H2O
isoform LeARG1, 2.2% of the activity with L-arginine, LeARG2, 3.6% of the activity with L-arginine
669279
Solanum lycopersicum
D-ornithine + urea
-
-
-
?
3.5.3.1
L-arginine + H2O
-
669279
Solanum lycopersicum
L-ornithine + urea
-
-
-
?
3.5.3.1
L-homoarginine + H2O
isoform LeARG1, 14% of the activity with L-arginine, LeARG2, 13% of the activity with L-arginine
669279
Solanum lycopersicum
L-2,6-diaminohexanoate + urea
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.1
?
x * 37000, SDS-PAGE and calculated; x * 37000, SDS-PAGE and calculated
Solanum lycopersicum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.1
7.5
-
;
Solanum lycopersicum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.1
7
12
no activity below or above this range; no activity below or above this range
Solanum lycopersicum
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.3.1
0.014
-
Nomega-hydroxy-L-arginine
pH 9.6, 37C; pH 9.6, 37C
Solanum lycopersicum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.1
2-mercaptopropionate
5 mM, isoform LeARG1, 38% residual activity, LeARG2, 38% residual activity
Solanum lycopersicum
3.5.3.1
3-Mercaptopropionate
5 mM, isoform LeARG1, 24% residual activity, LeARG2, 26% residual activity
Solanum lycopersicum
3.5.3.1
Mercaptoacetate
5 mM, isoform LeARG1, 28% residual activity, LeARG2, 26% residual activity
Solanum lycopersicum
3.5.3.1
Nomega-hydroxy-L-arginine
0.2 mM, isoform LeARG1, 7% residual activity, LeARG2, 5% residual activity
Solanum lycopersicum
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.5.3.1
0.014
-
Nomega-hydroxy-L-arginine
pH 9.6, 37C
Solanum lycopersicum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.1
37000
-
x * 37000, SDS-PAGE and calculated
Solanum lycopersicum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.3.1
leaf
induction of isoform LeARG2 upon wounding and treatment with jasmonic acid
Solanum lycopersicum
-
3.5.3.1
additional information
highest levels of enzyme activity in reproductive tissues
Solanum lycopersicum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.1
agmatine + H2O
isoform LeARG1, 0.5% of the activity with L-arginine, LeARG2, 0.6% of the activity with L-arginine
669279
Solanum lycopersicum
1,4-diaminobutane + urea
-
-
-
?
3.5.3.1
D-arginine + H2O
isoform LeARG1, 2.2% of the activity with L-arginine, LeARG2, 3.6% of the activity with L-arginine
669279
Solanum lycopersicum
D-ornithine + urea
-
-
-
?
3.5.3.1
L-arginine + H2O
-
669279
Solanum lycopersicum
L-ornithine + urea
-
-
-
?
3.5.3.1
L-homoarginine + H2O
isoform LeARG1, 14% of the activity with L-arginine, LeARG2, 13% of the activity with L-arginine
669279
Solanum lycopersicum
L-2,6-diaminohexanoate + urea
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.1
?
x * 37000, SDS-PAGE and calculated
Solanum lycopersicum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.1
7.5
-
-
Solanum lycopersicum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.1
7
12
no activity below or above this range
Solanum lycopersicum