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Literature summary extracted from

  • Kim, J.; Parker, J.S.; Murray, K.E.; Nibert, M.L.
    Nucleoside and RNA triphosphatase activities of Orthoreovirus transcriptase cofactor mu2 (2004), J. Biol. Chem., 279, 4394-4403.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.15 gene M1, expression of transcriptase cofactor my2 mutant K415A/K419A in Spodoptera frugiperda Sf21 insect cells using the baculovirus system mammalian orthoreovirus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.15 K415A/K419A site-directed mutagenesis, mutation of residues in the nucleotide binding domain of transcriptase cofactor my2, leads to loss of enzyme activity mammalian orthoreovirus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.15 0.00032
-
 RNA wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
3.6.1.15 2.5
-
 ATP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
3.6.1.15 3.7
-
 GTP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
3.6.1.15 3.8
-
 CTP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
3.6.1.15 9.4
-
 UTP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.15 Ca2+ activates mammalian orthoreovirus
3.6.1.15 Mg2+ activates mammalian orthoreovirus
3.6.1.15 Mn2+ activates mammalian orthoreovirus
3.6.1.15 additional information the enzyme activity depends on divalent cations and prefers Mg2+, Ca2+, and Mn2+ mammalian orthoreovirus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.15 RNA + H2O mammalian orthoreovirus removal of the gamma-phosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.15 mammalian orthoreovirus
-
 mORV
-
3.6.1.15 mammalian orthoreovirus mORV
-
 mORV
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.15 transcriptase cofactor my2 from host nucleoplasma by ion exchange and adsorption chromatography at room temperature mammalian orthoreovirus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.15 additional information the transcriptase cofactor my2 is associated to microtubules in host cells, overview mammalian orthoreovirus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.15 ATP + H2O
-
 mammalian orthoreovirus ADP + phosphate
-
 ?
3.6.1.15 ATP + H2O
-
 mammalian orthoreovirus mORV ADP + phosphate
-
 ?
3.6.1.15 CTP + H2O
-
 mammalian orthoreovirus CDP + phosphate
-
 ?
3.6.1.15 CTP + H2O
-
 mammalian orthoreovirus mORV CDP + phosphate
-
 ?
3.6.1.15 GTP + H2O
-
 mammalian orthoreovirus GDP + phosphate
-
 ?
3.6.1.15 GTP + H2O
-
 mammalian orthoreovirus mORV GDP + phosphate
-
 ?
3.6.1.15 additional information the virus core particles, an icosahedral multienzyme complex, and the transcriptase cofactor my2 show nucleoside triphosphatase activity mammalian orthoreovirus ?
-
 ?
3.6.1.15 additional information the virus core particles, an icosahedral multienzyme complex, and the transcriptase cofactor my2 show nucleoside triphosphatase activity mammalian orthoreovirus mORV ?
-
 ?
3.6.1.15 RNA + H2O removal of the gamma-phosphate mammalian orthoreovirus ?
-
 ?
3.6.1.15 UTP + H2O
-
 mammalian orthoreovirus UDP + phosphate
-
 ?
3.6.1.15 UTP + H2O
-
 mammalian orthoreovirus mORV UDP + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.15 22
-
 assay at room temperature mammalian orthoreovirus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.15 0.005
-
 RNA wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
3.6.1.15 7.3
-
 UTP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
3.6.1.15 8
-
 CTP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
3.6.1.15 9.8
-
 GTP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus
3.6.1.15 13.8
-
 ATP wild-type transcriptase cofactor my2, 22°C, 6.5-7.0 mammalian orthoreovirus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.15 6.5 7
-
 mammalian orthoreovirus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.15 5.7 9 50% of maximal activity at pH 5.7, and pH 9.0 mammalian orthoreovirus