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Literature summary extracted from

  • Sounni, N.E.; Roghi, C.; Chabottaux, V.; Janssen, M.; Munaut, C.; Maquoi, E.; Galvez, B.G.; Gilles, C.; Frankenne, F.; Murphy, G.; Foidart, J.M.; Noel, A.
    Up-regulation of vascular endothelial growth factor-A by active membrane-type 1 matrix metalloproteinase through activation of Src-tyrosine kinases (2004), J. Biol. Chem., 279, 13564-13574.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.24.80 medicine full enzymatic activity of MT1-MMP is required for a specific up-regulation of VEGF-A through an activation of Src tyrosine kinase pathways Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.80 MT1-MMP cDNA subcloned between the HindIII and EcoRI sites of pCDNA3.1 Zeo+ mammalian expression vector, annealed olignucleotides coding for huMT1-MMP signal sequence cloned upstream of ECFP in the NheI-AgeI sites of the pEGFP-C1 vector, amplified residues 283-582 of huMT1-MMP cloned into the BgIII-EcoRI sites of huMT1SSc1 in-frame with EGFP Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.80 C574S substitution in the cytoplasmic domain, reduction of pro-MMP2 activation, no up-regulation of VEGF expression Homo sapiens
3.4.24.80 E240A substitution in the active site, unable to activate exogenous pro-MMP2, fails to induce VEGF mRNA expression Homo sapiens
3.4.24.80 additional information partial deletion of the catalytic domain deltacd or the cytoplasmic tail delta577 of MT1-MMP, unable to activate exogenous pro-MMP2, no up-regulation of VEGF expression Homo sapiens
3.4.24.80 S577A substitution in the cytoplasmic domain, pro-MMP2-activating capacity not affected, similar VEGF upregulation Homo sapiens
3.4.24.80 T567A substitution in the cytoplasmic domain, pro-MMP2-activating capacity not affected, similar VEGF upregulation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.80 BB94 reduces the VEGF mRNA levels in MT clones but does not affect the basal VEGF mRNA levels in control clones Homo sapiens
3.4.24.80 genistein markedly suppresses the VEGF mRNA induction in MT clones without affecting the VEGF expression in control clones Homo sapiens
3.4.24.80 herbimycin-A strongly inhibits up-regulation effect of VEGF-A by MT1-MMP Homo sapiens
3.4.24.80 additional information TIMP-1 does not block up-regulation of VEGF-A by MT1-MMP, aprotinin, Pefabloc SC, leupeptin and pepstatin A fail to modulate the VEGF expression, wortmannin, PD98059 nor SB203580 affect MT1-MMP-mediated VEGF induction Homo sapiens
3.4.24.80 RO28-2653 reduces the VEGF mRNA levels in MT clones but does not affect the basal VEGF mRNA levels in control clones Homo sapiens
3.4.24.80 TIMP-2 blocks up-regulation of VEGF-A by MT1-MMP Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.80 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.80 MCF-7 cell
-
 Homo sapiens
-

Synonyms

EC Number Synonyms Comment Organism
3.4.24.80 membrane type 1 matrix metalloproteinase
-
 Homo sapiens
3.4.24.80 MT1-MMP
-
 Homo sapiens