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Literature summary extracted from

  • Mellroth, P.; Karlsson, J.; Steiner, H.
    A scavenger function for a Drosophila peptidoglycan recognition protein (2003), J. Biol. Chem., 278, 7059-7064.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.28 gene PGRP-SB1, expression of His6-tagged wild-type and mutant enzyme Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.28 C168A site-directed mutagenesis, the mutant is enzymatically inactive but retains its peptidoglycan affinity Drosophila melanogaster
3.5.1.28 C168S site-directed mutagenesis, the mutant is enzymatically inactive but retains its peptidoglycan affinity Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.28 teichoic acid
-
 Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.28 Zn2+ absolutely required for activity, mutant PGRP-SC1B lacking a potential zinc ligand is enzymatically inactive but retains its peptidoglycan affinity Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.28 N-acetylmuramoyl-L-alanine + H2O Drosophila melanogaster the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, the immunostimulatory properties of PGRP-SC1B-degraded peptidoglycan are highly reduced N-acetylmuramate + L-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.28 Drosophila melanogaster Q70PY2 gene PGRP-SB1 or CG9681; gene PGRP-SB1 or CG9681
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.28 gene PGRP-SB1, recombinant His6-tagged wild-type and mutant enzyme by nickel affinity chromatography Drosophila melanogaster

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.28 additional information
-
 the enzyme shows highest activity with peptidoglycan substrates from Staphylococcus aureus, and 34% and 23% of this activity with substrate from Micrococcus luteus and Bacillus megaterium, respectively, at pH 8.0 Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.28 additional information the enzyme does not show antibacterial activity Drosophila melanogaster ?
-
 ?
3.5.1.28 N-acetylmuramoyl-L-alanine + H2O the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, the immunostimulatory properties of PGRP-SC1B-degraded peptidoglycan are highly reduced Drosophila melanogaster N-acetylmuramate + L-alanine
-
 ?
3.5.1.28 N-acetylmuramoyl-L-alanine + H2O the enzyme performs cell wall lysis by cleavage of N-acetylmuramoyl-L-alanine bonds in dimeric cross-bridges that interlink the two murein strands in the peptidoglycan, peptidoglycan substrates from Staphylococcus aureus, Micrococcus luteus, and Bacillus megaterium, the enzyme hydrolyzes the lactylamide bond between the glycan strand and the cross-linking peptides, analysis of the cleavage products by mass spectrometry Drosophila melanogaster N-acetylmuramate + L-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.28 More the enzyme belongs to the peptidoglycan recognition protein, PGRP, family Drosophila melanogaster
3.5.1.28 PGRP-SB1
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.28 22 25 assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.28 7 8 assay at Drosophila melanogaster