Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Luberto, C.; Stonehouse, M.J.; Collins, E.A.; Marchesini, N.; El-Bawab, S.; Vasil, A.I.; Vasil, M.L.; Hannun, Y.A.
    Purification, characterization, and identification of a sphingomyelin synthase from Pseudomonas aeruginosa. PlcH is a multifunctional enzyme (2003), J. Biol. Chem., 278, 32733-32743.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.27 Mn2+ 4 mM, about 40% inhibition Pseudomonas aeruginosa
2.7.8.27 Ni2+ 2 mM, 75% inhibition Pseudomonas aeruginosa
2.7.8.27 tricyclodecan-9-yl-xanthogenate i.e. D609 Pseudomonas aeruginosa
2.7.8.27 Zn2+ 0.5 mM, about 95% inhibition Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.27 Pseudomonas aeruginosa
-
 strain PA01 and PAK
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.27
-
 Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.27 culture medium
-
 Pseudomonas aeruginosa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.27 additional information highly purified sphingomyelin synthase preparation also shows neutral sphingomyelinase activity Pseudomonas aeruginosa ?
-
 ?
2.7.8.27 phosphatidylcholine + 6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosine
-
 Pseudomonas aeruginosa N-hexanoyl-4-nitrobenz-2-oxa-1,3-diazole-sphingomyelin + 1,2-diacylglycerol
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.7.8.27 PlcH multifunctional enzyme Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.27 7.5 8.5
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.8.27 7 9.5 pH 7: about 50% of maximal activity, pH 9.5: 65% of maximal activity Pseudomonas aeruginosa