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Literature summary extracted from

  • Hemmerlin, A.; Rivera, S.B.; Erickson, H.K.; Poulter, C.D.
    Enzymes encoded by the farnesyl diphosphate synthase gene family in the big sagebrush Artemisia tridentata ssp. spiciformis (2003), J. Biol. Chem., 278, 32132-32140.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.67 gene FDS-5, cDNA library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of N-terminally His6-tagged enzyme in Escherichia coli strain XA90, expression of the fusion FDS-5 transit peptide-GFP protein in Nicotiana tabacum cv. xanthi cells with plastidial localization Artemisia spiciformis
2.5.1.69 gene FDS-5, cDNA library screening, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression of N-terminally His6-tagged enzyme in Escherichia coli strain XA90, expression of the fusion FDS-5 transit peptide-GFP protein in Nicotiana tabacum cv. xanthi cells with plastidial localization Artemisia spiciformis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.67 plastid
-
 Artemisia spiciformis 9536
-
2.5.1.69 plastid
-
 Artemisia spiciformis 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.67 Mg2+ required, 2 mM Artemisia spiciformis
2.5.1.69 Mg2+ required, 2 mM Artemisia spiciformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.67 2 dimethylallyl diphosphate Artemisia spiciformis
-
 diphosphate + chrysanthemyl diphosphate
-
 ?
2.5.1.69 2 dimethylallyl diphosphate Artemisia spiciformis a branching reaction diphosphate + lavandulyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.67 Artemisia spiciformis Q7XYS8 FDS-5 or ArtCDS; big sagebrush
-
2.5.1.69 Artemisia spiciformis Q7XYS8 big sagebrush
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.5.1.67 proteolytic modification the pre-enzyme contains an N-terminal plastidial targeting sequence Artemisia spiciformis
2.5.1.69 proteolytic modification the pre-enzyme contains an N-terminal plastidial targeting sequence Artemisia spiciformis

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.67 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XA90 by nickel affinity chromatography to over 95% purity Artemisia spiciformis
2.5.1.69 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain XA90 by nickel affinity chromatography to over 95% purity Artemisia spiciformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.67 2 dimethylallyl diphosphate
-
 Artemisia spiciformis diphosphate + chrysanthemyl diphosphate
-
 ?
2.5.1.67 2 dimethylallyl diphosphate a cyclopropanation Artemisia spiciformis diphosphate + chrysanthemyl diphosphate an irregular monoterpene product ?
2.5.1.67 2 dimethylallyl diphosphate reaction of EC 2.5.1.69, lavandulyl diphosphate synthase, a branching reaction Artemisia spiciformis diphosphate + lavandulyl diphosphate
-
 ?
2.5.1.67 isopentenyl diphosphate + dimethylallyl diphosphate reaction of EC 2.5.1.10, geranyltranstransferase, a chain elongation Artemisia spiciformis diphosphate + geranyl diphosphate
-
 ?
2.5.1.67 additional information multifunctional enzyme that catalyzes three competing isoprenoid condensation reactions chain elongation, cyclopropanation, and branching, substrate specificity, product analysis, overview Artemisia spiciformis ?
-
 ?
2.5.1.69 2 dimethylallyl diphosphate reaction of EC 2.5.1.67, chrysanthemyl diphosphate synthase, a cyclopropanation Artemisia spiciformis diphosphate + chrysanthemyl diphosphate an irregular monoterpene product ?
2.5.1.69 2 dimethylallyl diphosphate a branching reaction Artemisia spiciformis diphosphate + lavandulyl diphosphate
-
 ?
2.5.1.69 isopentenyl diphosphate + dimethylallyl diphosphate reaction of EC 2.5.1.10, geranyltranstransferase, a chain elongation Artemisia spiciformis diphosphate + geranyl diphosphate
-
 ?
2.5.1.69 additional information multifunctional enzyme that catalyzes three competing isoprenoid condensation reactions: chain elongation, cyclopropanation, and branching, substrate specificity, product analysis, overview Artemisia spiciformis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.67 CDS
-
 Artemisia spiciformis
2.5.1.67 FDS-5
-
 Artemisia spiciformis
2.5.1.69 CDS
-
 Artemisia spiciformis
2.5.1.69 FDS-5
-
 Artemisia spiciformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.67 37
-
 assay at Artemisia spiciformis
2.5.1.69 37
-
 assay at Artemisia spiciformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.67 7
-
 assay at Artemisia spiciformis
2.5.1.69 7
-
 assay at Artemisia spiciformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.67 6 9 pH profile Artemisia spiciformis
2.5.1.69 6 9 pH profile Artemisia spiciformis