EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.158 | gene CaMTL3, cDNA library construction, DNA and amino acid sequence determination and analysis, sequence comparison with other species | Coffea arabica |
2.1.1.159 | gene CaMTL2, cDNA library construction, DNA and amino acid sequence determination and analysis | Coffea arabica |
2.1.1.159 | gene CaMXMT, cDNA library construction, DNA and amino acid sequence determination and analysis, sequence comparison with other species, expression of CaMXMT as GST-fusion enzyme in Escherichia coli strain JM109 | Coffea arabica |
2.1.1.160 | gene CaMTL1, cDNA library construction, DNA and amino acid sequence determination and analysis, sequence comparison with other species | Coffea arabica |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.1.1.159 | cytoplasm | - |
Coffea arabica | 5737 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.158 | S-adenosyl-L-methionine + xanthosine | Coffea arabica | first step in caffeine biosynthesis pathway from xanthosine | S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
ir | |
2.1.1.159 | S-adenosyl-L-methionine + 7-methylxanthine | Coffea arabica | second step in caffeine biosynthesis pathway | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
2.1.1.159 | S-adenosyl-L-methionine + 7-methylxanthine | Coffea arabica | second step in caffeine biosynthesis pathway from xanthosine | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
2.1.1.160 | S-adenosyl-L-methionine + 3,7-dimethylxanthine | Coffea arabica | last step in caffeine biosynthesis pathway | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.158 | Coffea arabica | Q9AVK0 | gene CaMTL3, enzyme XMT1; var. caturra, two different N-methyltransferases XMT and MXMT | - |
2.1.1.159 | Coffea arabica | Q9AVJ9 | gene CaMXMT, enzyme MXMT1; var. caturra | - |
2.1.1.159 | Coffea arabica | Q9AVK1 | gene CaMTL2; var. caturra | - |
2.1.1.160 | Coffea arabica | Q9AVJ9 | gene CaMTL1; var. caturra | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.159 | recombinant GST-fusion enzyme from Escherichia coli strain JM109 by glutathione affinity chromatography | Coffea arabica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.158 | leaf | young and old leaves | Coffea arabica | - |
2.1.1.158 | root | - |
Coffea arabica | - |
2.1.1.158 | stem | - |
Coffea arabica | - |
2.1.1.159 | bud | - |
Coffea arabica | - |
2.1.1.159 | leaf | high expression level of MXMT in young leaves, low expression level in old leaves | Coffea arabica | - |
2.1.1.159 | leaf | old and young | Coffea arabica | - |
2.1.1.159 | additional information | wide tissue distribution | Coffea arabica | - |
2.1.1.159 | root | - |
Coffea arabica | - |
2.1.1.159 | root | low expression level of MXMT | Coffea arabica | - |
2.1.1.159 | stem | - |
Coffea arabica | - |
2.1.1.159 | stem | high expression level of MXMT | Coffea arabica | - |
2.1.1.160 | bud | - |
Coffea arabica | - |
2.1.1.160 | leaf | old and young | Coffea arabica | - |
2.1.1.160 | additional information | wide tissue distribution | Coffea arabica | - |
2.1.1.160 | root | - |
Coffea arabica | - |
2.1.1.160 | stem | - |
Coffea arabica | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.158 | S-adenosyl-L-methionine + xanthosine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
ir | |
2.1.1.158 | S-adenosyl-L-methionine + xanthosine | first step in caffeine biosynthesis pathway from xanthosine | Coffea arabica | S-adenosyl-L-homocysteine + 7-methylxanthosine | - |
ir | |
2.1.1.159 | S-adenosyl-L-methionine + 7-methylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
2.1.1.159 | S-adenosyl-L-methionine + 7-methylxanthine | second step in caffeine biosynthesis pathway | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
2.1.1.159 | S-adenosyl-L-methionine + 7-methylxanthine | second step in caffeine biosynthesis pathway from xanthosine | Coffea arabica | S-adenosyl-L-homocysteine + 3,7-dimethylxanthine | i.e. theobromine | ir | |
2.1.1.159 | S-adenosyl-L-methionine + paraxanthine | low activity | Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir | |
2.1.1.160 | S-adenosyl-L-methionine + 3,7-dimethylxanthine | - |
Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir | |
2.1.1.160 | S-adenosyl-L-methionine + 3,7-dimethylxanthine | last step in caffeine biosynthesis pathway | Coffea arabica | S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine | i.e. caffeine | ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.158 | 7-methylxanthosine synthase 1 | - |
Coffea arabica |
2.1.1.158 | N-methyltransferase | - |
Coffea arabica |
2.1.1.158 | xanthosine methyltransferase | - |
Coffea arabica |
2.1.1.158 | XMT1 | - |
Coffea arabica |
2.1.1.159 | 7-methylxanthine methyltransferase | - |
Coffea arabica |
2.1.1.159 | 7-methylxanthine N-methyltransferase | - |
Coffea arabica |
2.1.1.159 | MXMT | - |
Coffea arabica |
2.1.1.159 | MXMT1 | - |
Coffea arabica |
2.1.1.159 | N-methyltransferase | - |
Coffea arabica |
2.1.1.160 | caffeine synthase | - |
Coffea arabica |
2.1.1.160 | CS | - |
Coffea arabica |
2.1.1.160 | TCS1 | - |
Coffea arabica |