Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schulbach, M.C.; Mahapatra, S.; Macchia, M.; Barontini, S.; Papi, C.; Minutolo, F.; Bertini, S.; Brennan, P.J.; Crick, D.C.
    Purification, enzymatic characterization, and inhibition of the Z-farnesyl diphosphate synthase from Mycobacterium tuberculosis (2001), J. Biol. Chem., 276, 11624-11630.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.68 (1-[[(3E)-4,8-dimethylnona-3,7-dien-1-yl](hydroxy)phosphoryl]-1-ethylpropyl)phosphonic acid
-
 Mycobacterium tuberculosis
2.5.1.68 (E)-1-ethyl-1-[(4,8-dimethyl-3,7-nonadienyl)hydroxyphosphoryl] propyl phosphonic acid tripotassium salt
-
 Mycobacterium tuberculosis
2.5.1.68 (E)-geranyl diphosphate analogues design and synthesis of substrate analogue inhibitors, overview Mycobacterium tuberculosis
2.5.1.68 citronellyl diphosphate
-
 Mycobacterium tuberculosis
2.5.1.68 EDTA complete inhibition at 10 mM Mycobacterium tuberculosis
2.5.1.68 Mn2+ optimal at 0.1 mM, the enzyme absolutely requires a divalent cation, inhibition by Mn2+ at higher concentration of 5.0 mM Mycobacterium tuberculosis
2.5.1.68 additional information no inhibition by dipotassium salt of (E)-1-[(3,7-methyl-2,6-octadienyl)aminocarbonyl] propyl phosphonic acid, dipotassium salt of (E)-1-[[(3,7-methyl-2,6-octadienyl)oxy]aminocarbonyl] propyl phosphonic acid, and dipotassium salt of (E)-2-oxo-2-[(3,7-methyl-2,6-octadienyl)oxy-[amino]ethyl]phosphonic acid Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.68 0.016
-
 (E)-neryl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
2.5.1.68 0.038
-
 (E)-geranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
2.5.1.68 0.124
-
 isopentenyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.68 Mg2+ optimal at 1.0 mM, the enzyme absolutely requires a divalent cation Mycobacterium tuberculosis
2.5.1.68 Mn2+ optimal at 0.1 mM, the enzyme absolutely requires a divalent cation, inhibition by Mn2+ at higher concentration of 1.0-5.0 mM Mycobacterium tuberculosis
2.5.1.68 additional information no activation by Zn2+ and Ca2+ up to 5 mM Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.68 (E)-geranyl diphosphate + isopentenyl diphosphate Mycobacterium tuberculosis stereospecific reaction (2Z,6E)-farnesyl diphosphate + diphosphate
-
 ?
2.5.1.68 (E)-geranyl diphosphate + isopentenyl diphosphate Mycobacterium tuberculosis H37Rv stereospecific reaction (2Z,6E)-farnesyl diphosphate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.68 Mycobacterium tuberculosis
-
-
-
2.5.1.68 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.68 native enzyme in a single step by chitin affinity chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.68 additional information
-
-
 Mycobacterium tuberculosis
2.5.1.68 0.0007
-
 in presence of 1.0 mM Mg2+ Mycobacterium tuberculosis
2.5.1.68 0.0014
-
 in presence of 0.1 mM Mn2+ Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.68 (E)-geranyl diphosphate + isopentenyl diphosphate stereospecific reaction Mycobacterium tuberculosis (2Z,6E)-farnesyl diphosphate + diphosphate
-
 ?
2.5.1.68 (E)-geranyl diphosphate + isopentenyl diphosphate stereospecific reaction Mycobacterium tuberculosis H37Rv (2Z,6E)-farnesyl diphosphate + diphosphate
-
 ?
2.5.1.68 (E)-neryl diphosphate + isopentenyl diphosphate stereospecific reaction Mycobacterium tuberculosis (2Z,6Z)-farnesyl diphosphate + diphosphate
-
 ?
2.5.1.68 (E)-neryl diphosphate + isopentenyl diphosphate stereospecific reaction Mycobacterium tuberculosis H37Rv (2Z,6Z)-farnesyl diphosphate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.68 omega,E,Z-FPP
-
 Mycobacterium tuberculosis
2.5.1.68 Z-FPP synthase
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.68 7 8
-
 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.68 5.5 9.5
-
 Mycobacterium tuberculosis