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Literature summary extracted from

  • Schulbach, M.C.; Brennan, P.J.; Crick, D.C.
    Identification of a short (C15) chain Z-isoprenyl diphosphate synthase and a homologous long (C50) chain isoprenyl disphosphate synthase in Mycobacterium tuberculosis (2000), J. Biol. Chem., 275, 22876-22881.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.68 Rv1086, DNA and amino acid sequence determination and anaylsis, overexpression in Mycobacterium smegmatis cytosol and membranes, the transformed cells show a 5fold increased enzyme activity Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.68 Mg2+ activates Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.68 (E)-geranyl diphosphate + isopentenyl diphosphate Mycobacterium tuberculosis stereospecific reaction (2Z,6E)-farnesyl diphosphate + diphosphate
-
 ?
2.5.1.68 (E)-geranyl diphosphate + isopentenyl diphosphate Mycobacterium tuberculosis H37Rv stereospecific reaction (2Z,6E)-farnesyl diphosphate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.68 Mycobacterium tuberculosis
-
-
-
2.5.1.68 Mycobacterium tuberculosis H37Rv
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.68 0.00093
-
 recombinant Rv1086 in cytosol of Mycobacterium smegmatis Mycobacterium tuberculosis
2.5.1.68 0.00097
-
 recombinant Rv1086 in membranes of Mycobacterium smegmatis Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.68 (E)-geranyl diphosphate + isopentenyl diphosphate stereospecific reaction Mycobacterium tuberculosis (2Z,6E)-farnesyl diphosphate + diphosphate
-
 ?
2.5.1.68 (E)-geranyl diphosphate + isopentenyl diphosphate stereospecific reaction Mycobacterium tuberculosis H37Rv (2Z,6E)-farnesyl diphosphate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.68 More the enzyme contains a DD(XX)2D motif, secondary structure analysis Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.5.1.68 Rv1086
-
 Mycobacterium tuberculosis
2.5.1.68 short (C15) chain Z-isoprenyl diphosphate synthase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.68 37
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.68 7.9
-
 assay at Mycobacterium tuberculosis