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Literature summary extracted from

  • Spinelli, S.L.; Kierzek, R.; Turner, D.H.; Phizicky, E.M.
    Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast (1999), J. Biol. Chem., 274, 2637-2644.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.160 expression in Escherichia coli Mus musculus
2.7.1.160 expression in Escherichia coli Escherichia coli
2.7.1.160 expression in Escherichia coli Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.160 Escherichia coli
-
-
-
2.7.1.160 Mus musculus
-
-
-
2.7.1.160 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.160 by Blue Sepharose column chromatography Mus musculus
2.7.1.160 by Blue Sepharose column chromatography Escherichia coli
2.7.1.160 by nickel affinity chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.160 2'-phospho-[ligated tRNA] + NAD+ mTpt1 can catalyze the conversion of the KptA-generated intermediate to both product and the original substrate Mus musculus mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
 ?
2.7.1.160 2'-phospho-[ligated tRNA] + NAD+ production of a reaction intermediate, nicotinamide release occurs during intermediate formation, KptA can catalyze the conversion of the intermediate to both product and the original substrate Escherichia coli mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
 ?
2.7.1.160 2'-phospho-[ligated tRNA] + NAD+ Tpt1 can catalyze the conversion of the KptA-generated intermediate to both product and the original substrate Saccharomyces cerevisiae mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.160 KptA
-
 Escherichia coli
2.7.1.160 mTPT1
-
 Mus musculus
2.7.1.160 Tpt1
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.160 30
-
-
 Saccharomyces cerevisiae
2.7.1.160 37
-
-
 Mus musculus
2.7.1.160 37
-
-
 Escherichia coli